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Adenosine-enhanced ischemic preconditioning modulates necrosis and apoptosis: effects of stunning and ischemia–reperfusion  Bradford Stadler, BS, Jonathan.

Published byCorinne Claude Gagnon Modified over 6 years ago

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Presentation on theme: "Adenosine-enhanced ischemic preconditioning modulates necrosis and apoptosis: effects of stunning and ischemia–reperfusion  Bradford Stadler, BS, Jonathan."— Presentation transcript:

1 Adenosine-enhanced ischemic preconditioning modulates necrosis and apoptosis: effects of stunning and ischemia–reperfusion  Bradford Stadler, BS, Jonathan Phillips, BS, Yoshiya Toyoda, MD, Micheline Federman, PhD, Sidney Levitsky, MD, James D McCully, PhD  The Annals of Thoracic Surgery  Volume 72, Issue 2, Pages (August 2001) DOI: /S (01)

2 Fig 1 Infarct size expressed as a percent of area at risk after 15, 30, and 60 minutes of regional ischemia and 120 minutes of reperfusion in regional ischemia (RI), adenosine only (ADO), ischemic preconditioning (IPC), and adenosine-enhanced ischemic preconditioning (APC) hearts. Results are shown as the mean ± standard error of the mean for n = 6 at each time point for all groups. No significant differences in area at risk were observed within or among experimental groups. ∗p < 0.05 versus RI; ∗∗p < 0.05 versus RI, ADO, and IPC. The Annals of Thoracic Surgery  , DOI: ( /S (01) )

3 Fig 2 Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)–positive cells/3,000 myocytes after 15, 30, and 60 minutes of regional ischemia of the left anterior descending coronary artery and 120 minutes of reperfusion in regional ischemia (RI), adenosine only (ADO), ischemic preconditioning (IPC), and adenosine-enhanced ischemic preconditioning (APC) hearts. Results are shown as the mean ± standard error of the mean of 10 to 15 high-powered (20×) fields in 5 to 6 slides from the six samples at each time for each group. ∗p < versus RI; ∗∗p < versus IPC. The Annals of Thoracic Surgery  , DOI: ( /S (01) )

4 Fig 3 Representative terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling photomicrographs of regional ischemia, adenosine only (ADO), ischemic preconditioning (IPC), and adenosine-enhanced ischemic preconditioning (APC) sections from ischemic zone after 60 minutes of regional ischemia and 120 minutes of reperfusion. Control zone samples from RI hearts after 60 minutes of regional ischemia and 120 minutes of reperfusion are shown for comparative purposes. TUNEL–positive cells were visualized by indirect digoxigenin fluorescence labeling of DNA with propidium iodide (red), fluorescein isothiocyanate (FITC, green), and dual propidium iodide/fluorescein isothiocyanate filters. TUNEL–positive cells are shown as green in fluorescein isothiocyanate and yellow under propidium iodide/fluorescein isothiocyanate filter. Cardiac troponin I was used for the identification of myocytes. The Annals of Thoracic Surgery  , DOI: ( /S (01) )

5 Fig 4 Representative agarose gels of DNA from regional ischemia (RI), adenosine only (ADO), ischemic preconditioning (IPC), and adenosine-enhanced ischemic preconditioning (APC) hearts after 15, 30, and 60 minutes of regional ischemia and 120 minutes of reperfusion. DNA (5 μg) was fractionated on a 1.8% agarose gel in 40 mmol/L Tris-acetate and 2 mmol/L EDTA, using modified Tris acetate-EDTA loading buffer that excluded dye markers, and then stained with ethidium bromide. Molecular weight markers (100 base pairs) are shown on the left of each gel. Control zone DNA from RI and APC hearts after 60 minutes of regional ischemia and 120 minutes of reperfusion are shown for comparative purposes. DNA oligonucleotide ladder formation (multimers of approximately 200 base pairs) are evident in RI, ADO, and IPC hearts after 60 minutes of regional ischemia and 120 minutes of reperfusion. No oligonucleotide ladders were observed in any APC samples or in any control region. The Annals of Thoracic Surgery  , DOI: ( /S (01) )

6 Fig 5 Representative photomicrographs of hematoxylin and eosin–stained sections from regional ischemia, adenosine only (ADO), ischemic preconditioning (IPC), and adenosine-enhanced ischemic preconditioning (APC) hearts after 60 minutes of regional ischemia of the left anterior descending coronary artery and 120 minutes of reperfusion (ischemic zone). Control zone samples from regional ischemia and APC hearts after 60 minutes of regional ischemia and 120 minutes of reperfusion are shown for comparative purposes. No ultrastructural differences within or among groups could be observed. The Annals of Thoracic Surgery  , DOI: ( /S (01) )

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