1. Ocular defects produced by Daam1 mutation and in combination with diabetes.
Ocular defects produced by Daam1 mutation and in combination with diabetes. Adult Daam1+/gt mutant with an eye defect indicated by the white double arrow (A). At E12.5, Daam1+/+ embryo with a normal eye (B), Daam1+/gt embryo with microphthalmia (D) and a Daam1gt/gt embryo with microphthalmia (F). Corresponding transverse paraffin sections stained with haematoxylin and eosin (C,E,G). The proportion of total eye defects differs significantly between Daam1+/+ (adults, 0/64; embryos, 0/43) and Daam+/gt/Daam1gt/gt mice (adult Daam1+/gt, 3/42; Daam1+/gt embryos, 2/66; Daam1gt/gt embryos, 1/19; Fisher’s exact test, P=0.033). At E12, Daam1+/+ embryo from a control dam with normal eyes (H). At E12, Daam1+/gt embryo from a control dam with microphthalmia (J). At E11, Daam1+/+ embryo exposed to maternal diabetes with anophthalmia (L). At E12, Daam1+/gt embryo exposed to maternal diabetes with anophthalmia (N). The corresponding transverse paraffin sections stained with haematoxylin and eosin (I,K,M,O). The main eye structures are indicated: L, Lens; optic nerve (black arrow); optic stalk (white arrowhead); optic cup (white arrow); neural retina (asterisk); retinal pigmented epithelium (black arrowhead). Scale bars: 1000 μm (B, also applies to D,F,H,J,L,N); 100 μm (C, also applies to E,G,I,K,M,O).
Beatriz López-Escobar et al. Dis. Model. Mech. 2015;8:
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