1. Design Automation for Flow-based Microfluidic BioChips
Jeffery McDaniel
Brian Crites
Christopher Curtis
Philip Brisk

2. Mars Organic Analyzer
[Skelly et al. PNAS 2005]

3. BioCoder (Original Language)

4. BioCoder (Updated Language)

5. Microfluidic Hardware Design Language (MHDL)

6. Flow Layer: Placement Planar Embedding

7. Flow Layer: Placement Planar Embedding

8. Flow Layer: Placement Planar Embedding

9. Flow Layer: Placement Planar Embedding

10. Flow Layer: Placement Planar Embedding

11. Flow Layer Routing: Network Flow Model

12. Flow Layer Routing: Network Flow Model

13. Layer Routing Network Flow Model

14. Flow Layer Routing: Post Processing

15. Performance Trace /* Execution Trace */
L1 is filled with 240 cubic microns of PCR Reagents
L2 is filled with 600 cubic microns of DNA
L3 is filled with 500 cubic microns of RDReagents
B4 is filled with 100 cubic microns of ReproGel
Moved 240 cubic microns from L1 to PCR_chamber
L1->L1V1( )->V1(0)->V1V3( )->V3(0)->
V3V4( )->V4(0)->PCR_chamber
L1 is now empty
PCR_chamber now contains 240 cubic microns of
PCR_Reagents
Time: 891 ms
...
/* Remaining Fluids */
PCR_chamber contains 300 cubic microns of DNA RD_chamber contains 150 cubic microns of DNA
/* Statistics Tracking */ Total Valve Actuation Time: Total Time in Lines: Total Transfer Time: Total Time in Components: Total Time: Total Number of Valve Actuations:
2400ms ms 5224ms e+06ms e+06ms 17

16. Valve Actuation Sequence

17. Conclusion BioCoder Compiler MHDL Compiler Placement and Routing
High-level language for assay specification
MHDL Compiler
Continuous Fluid Flow LoC Specification
Verification and Simulation framework
Placement and Routing
Planar Embedding and Expansion
Network Flow Channel Routing