1. July 13, 2004 Summarized by Ji-Yoon Park
Methods for Manipulating DNA Molecules in a Micrometer Scale using Optical Techniques
July 13, 2004
Summarized by Ji-Yoon Park

2. Abstract Optical method
Control the position & reaction of DNA molecules in a micrometer scale
Two single laser beams for optical manipulation & temperature control
Independent control

3. Introduction Optical computing In this paper
Using inherent property of light
Fast propagation, parallelism, and a large bandwidth
Example:
Vertical cavity surface-emitting lasers (VCSELs)
Diffractive optical elements (DOEs)
DNA & Light: high-performance information system
In this paper
Methods for controlling the position & reaction of DNA in micrometer scale

4. Optically Assisted DNA Computing
Figure 1. The basic concept of optically assisted DNA computing

5. Manipulation of DNA Optical manipulation
A method for non-invasively manipulating an object with a radiation pressure force induced between light and the object
DNA cluster
DNA strands connected to a microscopic bead

6. Manipulation of DNA using Optical Techniques
Figure 2. A method for controlling the temperature of a solution in a micrometer scale

7. Experimental Section (1/2)
Three step operation
Attaching the DNA cluster to the bead
Translation of the bead
Detaching the DNA cluster from the bead

8. Experimental Section (2/2)
Anti-tag sequence immobilization to bead (diameter: 6 µm) ’-biotin-CATAG TACAA ATCTT ACCTC ATTTC AGTTA CTGAT CCACG-3’
Alexa Fluor 647 attach the tag sequence
5’-Alexa 647- CGTGG ATCAG TAACT GAAAT GAGGT AAGAT TTGTACTATG-3’
Hybridization with anti-tag sequence and tag sequence
Extraction of DNA cluster
Substrate coating with titanylphthalocyanine (0.15 µm) & gold deposition
Thiol-modified anti-tag sequence binding to substrate

9. Experimental Setup
Figure 3. Experimental setup

10. Figure 4. Experimental result of detachment of information DNA from a substrate

11. Dependence of the Fluorescence Intensity
Figure 5. Dependence of the fluorescent intensity on irradiation cycle when the laser beam for temperature control is used (i) fluorescent molecules are attached to tag DNA (ii) Bead on the anti-tag DNA with fluorescent molecules
Irradiation cycle: 5 mW for 15 sec & stop for 4 sec

12. Fluorescence Images
Figure 6. Experimental result of translation and detachment of DNA (a) at initial state (b) after translation (c) after detachment: 3 mW for 1 min

13. Fluorescence Intensity of Microwell Array
anti-tag DNA attachment
Tag DNA binding
Irradiation (4 mW for 1 min)
Fluorescence intensity
Figure 7. Fluorescent intensity of a DNA cluster (a) before and (b) after irradiating with a laser beam

14. Conclusions
Develop methods for manipulating DNA molecules in a micrometer scale with optical techniques
The position and reaction of DNA are controlled independently using two laser beams with different wavelengths