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Circulating anti-β2-glycoprotein I antibodies of peripheral arterial disease patients trigger a genomic overexpression of Toll-like receptor 4 in endothelial.

Published byFrançois-Xavier Croteau Modified over 5 years ago

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Presentation on theme: "Circulating anti-β2-glycoprotein I antibodies of peripheral arterial disease patients trigger a genomic overexpression of Toll-like receptor 4 in endothelial."— Presentation transcript:

1 Circulating anti-β2-glycoprotein I antibodies of peripheral arterial disease patients trigger a genomic overexpression of Toll-like receptor 4 in endothelial cells  Cesar Varela, MD, Joaquin de Haro, MD, Silvia Bleda, MD, Javier Rodriguez-Padilla, MD, Antonio Ferruelo, PhD, Francisco Acín, MD, PhD  Journal of Vascular Surgery  Volume 61, Issue 4, Pages e1 (April 2015) DOI: /j.jvs Copyright © 2015 Society for Vascular Surgery Terms and Conditions

2 Fig 1 Toll-like receptor (TLR) cellular signaling cascade. ABGPI, Anti-β2-glycoprotein I; B2GPI, β2-glycoprotein I; IL-1, interleukin-1; IL-1R, interleukin-1 receptor; IRAKs, IL-1R-associated kinases; MyD88, myeloid differentiation primary response gene 88; NF-κB, nuclear factor-κB; RQ, relative quantification; TIR domain, Toll/IL-1R homologous domain; TLR2, Toll-like receptor 2; TLR4, Toll-like receptor 4. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

3 Fig 2 A, Toll-like receptor 4 (TLR4) expression by human aortic endothelial cell (HAEC) culture after stimulation with serum from peripheral arterial disease (PAD) patients and healthy controls without PAD. B, TLR4 expression by HAECs according to anti-β2-glycoprotein I (ABGPI) antibody titers. Group I, PAD and ABGPI titer ≥1:100; group II, PAD and ABGPI titer <1:100; RQ, relative quantification. Variables are expressed as mean ± standard deviation (error bars). *Indicates statistically different bars and statistical trends. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

4 Fig 3 A, Toll-like receptor 2 (TLR2) expression by human aortic endothelial cell (HAEC) culture after stimulation with serum from peripheral arterial disease (PAD) patients and healthy controls without PAD. B, TLR2 expression by HAEC culture according to anti-β2-glycoprotein I (ABGPI) antibody titers. Group I, PAD and ABGPI titer ≥1:100; group II, PAD and ABGPI titer <1:100; RQ, relative quantification. Variables are expressed as mean ± standard deviation (error bars). *Indicates statistically different bars and statistical trends. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

5 Fig 4 A, Myeloid differentiation primary response gene 88 (MyD88) expression by human aortic endothelial cell (HAEC) culture is shown after stimulation with serum from peripheral arterial disease (PAD) patients and healthy controls without PAD. B, MyD88 expression by HAEC culture according to anti-β2-glycoprotein I (ABGPI) antibody titers. Group I, PAD and ABGPI titer ≥1:100; group II, PAD and ABGPI titer <1:100; RQ, relative quantification. Variables are expressed as mean ± standard deviation (error bars). *Indicates statistically different bars and statistical trends. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

6 Fig 5 A, Interleukin-1 receptor-associated kinase 1 (IRAK1) expression by human aortic endothelial cell (HAEC) culture after stimulation with serum from peripheral arterial disease (PAD) patients and healthy controls without PAD. B, IRAK1 expression by HAEC culture according to anti-β2-glycoprotein I (ABGPI) antibody titers. Group I, PAD and ABGPI titer ≥1:100; group II, PAD and ABGPI titer <1:100; RQ, relative quantification. Variables are expressed as mean ± standard deviation (error bars). *Indicates statistically different bars and statistical trends. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

7 Supplementary Fig (online only)
A, Correlation between Toll-like receptor (TLR4) and Toll-like receptor 2 (TLR2) genomic expression. B, Correlation between TLR4 and myeloid differentiation primary response gene 88 (MyD88) genomic expression. C, Correlation between MyD88 and IRAK1 genomic expression. RQ, Relative quantification. Journal of Vascular Surgery  , e1DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

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