1. Red cells from glutathione peroxidase-1–deficient mice have nearly normal defenses against exogenous peroxides
by Robert M. Johnson, Gerard Goyette, Yaddanapudi Ravindranath, and Ye-Shih Ho
Blood
Volume 96(5):
September 1, 2000
©2000 by American Society of Hematology

2. Fluorescence intensity of 50 μmol/L cis-parinaric acid in a 2 mL suspension of red cells, 10% hematocrit, in PBS with 10 mmol/L glucose and 50 μg/mL gentamycin.Readings were taken every 15 seconds.
Fluorescence intensity of 50 μmol/L cis-parinaric acid in a 2 mL suspension of red cells, 10% hematocrit, in PBS with 10 mmol/L glucose and 50 μg/mL gentamycin.Readings were taken every 15 seconds. After 150 seconds of equilibration, either 50 μL cumene hydroperoxide in ethanol was added (100 μmol/L final concentration) (filled symbols), or 50 μL ethanol (open symbols) was added to controls. There was no difference between the rate of fluorescence loss in wild-type red cells (circles) and GSHPx-1–deficient red cells (squares).Two runs are shown.
Robert M. Johnson et al. Blood 2000;96:
©2000 by American Society of Hematology

3. Catalase inactivation (A) and hemoglobin oxidation (B) in red cells exposed to 50 mmol/L 3-AT and a continuous flux of H2O2.Hematocrit 10%, Krebs-Ringer buffer.
Catalase inactivation (A) and hemoglobin oxidation (B) in red cells exposed to 50 mmol/L 3-AT and a continuous flux of H2O2.Hematocrit 10%, Krebs-Ringer buffer. The rate of H2O2 production was 0.123μmol/L per minute (circles), μmol/L per minute (squares), and 1.23 μmol/L per minute (triangles). Solid lines, wild-type red cells; dashed lines, GSHPx-1–deficient red cells.
Robert M. Johnson et al. Blood 2000;96:
©2000 by American Society of Hematology

4. Hemoglobin oxidation as a function of catalase inactivation
Hemoglobin oxidation as a function of catalase inactivation.The percentage methemoglobin in GSHPx-1–deficient cells is plotted versus the residual catalase activity (percentage of starting activity).
Hemoglobin oxidation as a function of catalase inactivation.The percentage methemoglobin in GSHPx-1–deficient cells is plotted versus the residual catalase activity (percentage of starting activity).
Robert M. Johnson et al. Blood 2000;96:
©2000 by American Society of Hematology