Presentation is loading. Please wait.

Presentation is loading. Please wait.

Directing actin polymerization to membranes

Published byKelly Sherman Modified over 6 years ago

Similar presentations

Presentation on theme: "Directing actin polymerization to membranes"— Presentation transcript:

1 Directing actin polymerization to membranes
MRSEC 2017 C.F. Kelley, A.A. Rodal, Brandeis University Biological membranes are deformed and shaped by proteins that assemble into higher-order scaffolds. These scaffolds target the force-generating polymerization of actin filaments to deform and shape the membrane. MRSEC experiments show that membrane-remodeling proteins can be maintained in an autoinhibited state, preventing spurious actin polymerization in solution. Release from autoinhibition by multiple coincident inputs ensures that force is generated only at the membrane. These principles can be applied to nanoparticles and engineered proteins to shape membranes in a temporally and spatially controlled fashion. Cell-sized water droplets emulsified in decane/lipid F-BAR (SNAP549) Deregulated Activated Actin polymerization at membranes In solution [Nwk-SNAP549]=500nM, [Dap160SH3CD]=2uM [WASp]=50nM [Arp2/3]=50nM [Actin] = 2uM (10% Oregon Green)

Download ppt "Directing actin polymerization to membranes"

Similar presentations

Cellular Transport.

Cellular Transport.
ODEs part 3. Network model example Model considers positive feedback loops involving signalling pathways and/or gene regulation. Multiple interlinked.

ODEs part 3. Network model example Model considers positive feedback loops involving signalling pathways and/or gene regulation. Multiple interlinked.
The Cell Part 3.

The Cell Part 3.
Animal Form & Function Physiology AP Biology. Nerve Impulse Transmission  Resting potential  More negative inside cell than outside Why? Large negatively.

Animal Form & Function Physiology AP Biology. Nerve Impulse Transmission  Resting potential  More negative inside cell than outside Why? Large negatively.
Www.soran.edu.iq Cell and Molecular Biology Behrouz Mahmoudi Cytoskeleton-1 1.

Cell and Molecular Biology Behrouz Mahmoudi Cytoskeleton-1 1.
OSMOSIS OSMOSIS: The diffusion of water through a selectively permeable membrane SELECTIVELY PERMEABLE: some substances pass like water and others do.

OSMOSIS OSMOSIS: The diffusion of water through a selectively permeable membrane SELECTIVELY PERMEABLE: some substances pass like water and others do.
Membranes in Biology Rob Phillips California Institute of Technology (McMahon)

Membranes in Biology Rob Phillips California Institute of Technology (McMahon)
Inside of cell Outside of cell Carrier proteins allow glucose to enter a cell at a faster rate than would be possible by simple diffusion across the membrane.

Inside of cell Outside of cell Carrier proteins allow glucose to enter a cell at a faster rate than would be possible by simple diffusion across the membrane.
Lecture 2 Rob Phillips California Institute of Technology (Block et al.) (Wuite et al.)

Lecture 2 Rob Phillips California Institute of Technology (Block et al.) (Wuite et al.)
Chapter 16 The Cytoskeleton. Cell stained with general protein stain shows the cytoskeleton.

Chapter 16 The Cytoskeleton. Cell stained with general protein stain shows the cytoskeleton.
The Chapter 4 Concept Check Questions are due on Monday. The Chapter 4 Test is on Monday. The T-Shirt vote will be on Tuesday. Homework: Eukaryopolis -

The Chapter 4 Concept Check Questions are due on Monday. The Chapter 4 Test is on Monday. The T-Shirt vote will be on Tuesday. Homework: Eukaryopolis -
Cytoskeleton Introduction The cytoskeleton of a cell provides structure, strength, and motility. - It provides a cellular scaffolding upon which the cellular.

Cytoskeleton Introduction The cytoskeleton of a cell provides structure, strength, and motility. - It provides a cellular scaffolding upon which the cellular.
AP Biology 2007-2008 Tour of the Cell Part 2 AP Biology From food to making Energy  Cells must convert incoming energy to forms that they can use for.

AP Biology Tour of the Cell Part 2 AP Biology From food to making Energy  Cells must convert incoming energy to forms that they can use for.
2.4.1 Draw and label a diagram to show the structure of membranes (1).

2.4.1 Draw and label a diagram to show the structure of membranes (1).
Cell Transport & Homeostasis Key Terms

Cell Transport & Homeostasis Key Terms
The Dependence of Bacterial Cell Growth on Turgor Pressure Rico Rojas.

The Dependence of Bacterial Cell Growth on Turgor Pressure Rico Rojas.
Water movement through membranes Plant Physiology UNI 2009.

Water movement through membranes Plant Physiology UNI 2009.
Homeostasis and Cell Transport

Homeostasis and Cell Transport
Membranes Phospholipids and Bilayers. What do cell membranes consist of? Experiment: ABCDEF 10 cc of milk 10 cc of vinegar 10 cc of oil 10 cc of vinegar.

Membranes Phospholipids and Bilayers. What do cell membranes consist of? Experiment: ABCDEF 10 cc of milk 10 cc of vinegar 10 cc of oil 10 cc of vinegar.
Energy Energy (E)– the ability to perform work Potential E (E P )- stored energy Kinetic E (E K )– energy of motion Heat – kinetic energy of molecules.

Energy Energy (E)– the ability to perform work Potential E (E P )- stored energy Kinetic E (E K )– energy of motion Heat – kinetic energy of molecules.

Similar presentations

Ads by Google