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Scientific Method 1. STEPS Problem Research Hypothesis
Procedure / Data Results / Conclusion
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2. Hypothesis Educated guess / a “prediction” based on your research
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3. Independent Variable What “I” control, as the experimentor
Sometimes called “Manipulated Variable”
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4. Dependent Variable Changes “on its own”, as a RESULT of the experiment “Responding” Variable ALWAYS graphed on Y-axis
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5. Constants/Controls Constants: what keep the SAME
Control: does NOT receive the I.V. (for COMPARISON)
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Effect of Salt on Growth
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Effect of Salt on Growth
DV 5 10 IV % SALT
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6. Validity/Reliability
VALID: measures what it says it measures RELIABLE: can repeat it & get the same results
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7. DATA Range: Greatest – Lowest Mean: Average
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8. Metrics Mass: Gram Volume: Liter Distance: Meter
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9. Water Structure + + + + Hydrogen Bond + - - _ - - +
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10. Water Properties A POLAR molecule!!
A. Form HYDROGEN bonds, making water COHESIVE and ADHESIVE B. Water ABSORBS heat, stabilizing land & sea TEMPERATURES
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10. Water Properties C. Evaporates … rids of excess HEAT
D. Ice FLOATS, preventing LAKES from freezing SOLID E. Universal SOLVENT (can DISSOLVE many substance)
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11. BONDING A. IONIC: + to – (ex: Na+Cl- or salt)
B. COVALENT: share ELECTRONS (ex: H2O) C. HYDROGEN: bonds between MOLECULES, such as WATER
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12. Water DISSOCIATES into H+ and OH- ions, measured as pH
H H OH-
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pH SCALE Neutral 14 7 Basic Acidic Neutral = 7 Acidic Ranges = 0 – 6
7 Basic Acidic Neutral = 7 Acidic Ranges = 0 – 6 Basic Ranges =
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14. Importance of pH Cells & enzymes only tolerate SMALL CHANGES in pH….. Every cell/enzyme functions best within a narrow RANGE Acidic-loving grows best between: 0 – 6 Neutral-loving best at/near: 7
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15. CELL MEMBRANE
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15. Cell Membrane Selective Permeability = Lets some things in, not others Help maintain HOMEOSTASIS (term for keeping everything in BALANCE)
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CELL TRANSPORT DIFFUSION: movement of materials from HIGH conc. To LOW conc. OSMOSIS: WATER (from H L) ACTIVE TRANSPORT: uses ENERGY (can go from L H)
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16. ELEMENTS Remember the “CHNOPS” !! H C O N P S
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17. ENZYMES ….they serve as CATALYSTS ….they SPEED UP reactions without being CHANGED or USED UP Enzymes are PROTEINS (contain C – N bonds)
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17. ENZYMES Active Site: where enzyme attaches to the substrate
Substrate: molecule that is changed by the enzyme Product: RESULT of the enzyme-substrate reaction “Lock & Key” = specific enzyme for a specific substrate
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17. ENZYMES
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18. MACROMOLECULES CARBOHYDRATES LIPIDS PROTEINS NUCLEIC ACIDS
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CARBS: ELEMENTS C, H, O Sucrose (endings in “ose”) Polysaccharides
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Carbs: Buiding Blocks Repeating SUGAR units:
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Carbs: Function/Importance
ENERGY (through Respiration – ATP) STRUCTURE (as Cellulose in Plant cell walls)
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LIPIDS: Elements C, H, O, sometimes P (if in cell membrane Oils Waxes
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Lipids: Building Blocks
Glycerol + Fatty Acids: CH2
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LIPIDS: Function/Importance
CELL MEMBRANES! Long-term energy storage Insulation
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Proteins: Elements C, H, O, and N Enzymes Muscles Cell Structure
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Proteins: Building Blocks
Built of Repeating AMINO ACID units Also called POLYPEPTIDES
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Proteins: Function/Importance
Structural foundation of ALL CELLS ENZYMES – act as catalysts
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Nucleic Acids: Elements
C, H, O, N, P DNA RNA
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Nucleic Acids: Buidling Blocks
NUCLEOTIDES: SUGAR + BASE + Phosphate
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Nucleic Acids: Functions
Store genetic information (DNA) Carry instructions to the ribosomes (mRNA)
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A. Dissecting B. Compound Light C. Electron Scanning
20. MICROSCOPES A. Dissecting B. Compound Light C. Electron Scanning TOTAL MAGNIFICATION = Obj. Lense X Eyepiece
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21. CELL THEORY A. All living things are made of cells
B. Cells = basic unit of structure and function C. Cells come from preexisting cells
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Prokaryote (no nucleus) Eukaryote (have nucleus & organelles)
22. Types of Cells Prokaryote (no nucleus) Eukaryote (have nucleus & organelles)
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Nucleus Mitochondria Chloroplasts Golgi, E.R. Vacuoles, etc.
EUKARYOTE Cells Nucleus Mitochondria Chloroplasts Golgi, E.R. Vacuoles, etc.
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DNA / RNA Cell Membrane Cytoplasm Ribosomes Cytoskeleton
22. BOTH Cell Types Have: DNA / RNA Cell Membrane Cytoplasm Ribosomes Cytoskeleton
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PROKARYOTE Cells: NO Nucleus NO Organelles Bacteria Kingdoms
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Cell Organelles NUCLEUS: control center – DNA, chromosomes, genes
CELL MEMBRANE: surrounds cell; bilipid layer; controls in & out CYTOPLASM: area between cell membrane & nucleus
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CELL ORGANELLES GOLGI APPARATUS: finishing/packaging of molecules
CHLOROPLASTS: site of photosynthesis (green) VACUOLES: store/transport wastes, water, or food
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CELL ORGANELLES RIBOSOMES: site of PROTEIN SYNTHESIS!!!
MITOCHONDRIA: powerhous of cell; site of respiration (ATP) ENDOPLASMIC RETICULUM (ER): transports; “rough” has ribosomes
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CELL ORGANELLES CENTRIOLES: assist in cell division LYSOSOMES: digestive enzymes (“suicide sacs”) NUCLEOLUS: in nucleus; produces RNA
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19. Photosynthesis: Inputs
Chlorophyll + Sun + CO2 + Water
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19. Respiration: inputs SUGAR + O2 + Enzymes
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19. Products (Photosyn.) SUGARS OXYGEN
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19. Products: Respiration
ATP (energy) CO2 H2O
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SITE: Photosynthesis CHLOROPLAST
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SITE: Respiration MITOCHONDRIA
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RATE: Photosynthesis Amounts of: Light CO2 Water Temperature
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RATE: Respiration Amounts of: Sugar Oxygen Enzymes
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19.A “Light Reaction” or… Photochemical Reaction = H2O + SUN H+ + O2
B. “Dark Reaction” or … Carbon Fixation = H CO SUGARS
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19C. RESPIRATION: can be either AEROBIC (with oxygen) or ANAEROBIC (without oxygen) -- look for LACTIC ACID as a product
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25. STAGES of Cell Division
A. MITOSIS: 2 identical daughter cells (each 2n)
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25. Cell Division: MEIOSIS
B. MEIOSIS: 4 haploid sex cells
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26. Mitosis V. Meiosis Mitosis Meiosis Cell division Cell division
ALL cells Sex cells only IPMAT once IPMAT twice 2 diploid cells 4 haploid cell 2n 2n + 2n 2n n+n+n+n
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REPRODUCTION Sexual: union of male & female gametes
Asexual: producing a copy WITHOUT forming gametes
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