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A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial.

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Presentation on theme: "A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial."— Presentation transcript:

1 A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence  L. Pérez-Lago, S. Izco, M. Herranz, G. Tudó, M. Carcelén, I. Comas, O. Sierra, J. González-Martín, M.J. Ruiz-Serrano, J. Eyene, E. Bouza, D. García de Viedma  Clinical Microbiology and Infection  Volume 23, Issue 2, Pages (February 2017) DOI: /j.cmi Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

2 Fig. 1 (a) Amplification patterns obtained from EG-MDR strain-specific multiplex ASO-PCR results for control EG-MDR and non-EG-MDR strains. Amplicons corresponding to each of four targeted SNPs are indicated. (b) Selection of EG-MDR and non-EG-MDR strains. ASO, allele-specific oligonucleotide; EG-MDR, multidrug-resistant prevalent tuberculosis strain in Equatorial Guinea; MW, 100 bp ladder; SNP, single nucleotide polymorphism. Clinical Microbiology and Infection  , 92-97DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

3 Fig. 2 Results obtained after applying EG-MDR strain-specific multiplex ASO-PCR to selection of historical isolates. Isolates identified as EG-MDR or non-EG-MDR are indicated. ASO, allele-specific oligonucleotide; EG-MDR, multidrug-resistant prevalent tuberculosis strain in Equatorial Guinea; MW, 50 bp ladder. Clinical Microbiology and Infection  , 92-97DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions


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