1. Volume 152, Issue 1, Pages 120-131 (January 2013)
Human TFIID Binds to Core Promoter DNA in a Reorganized Structural State 
Michael A. Cianfrocco, George A. Kassavetis, Patricia Grob, Jie Fang, Tamar Juven-Gershon, James T. Kadonaga, Eva Nogales 
Cell 
Volume 152, Issue 1, Pages (January 2013)
DOI: /j.cell
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2. Cell  , DOI: ( /j.cell )
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3. Figure 1
Lobe A Exists in a Range of Positions Relative to a Stable BC Core within the Purified TFIID Complex
2D reference-free cryo-EM class averages of TFIID are shown alongside contour models to indicate lobe positions within each average. Lobe A is colored yellow and the stable BC core is colored blue. Scale bar in (A) is 200 Å. See also Figure S1 and Movie S1.
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4. Figure 2
TFIID’s Conformational Landscape Changes in Response to TFIIA and SCP DNA
(A–D) Distribution of lobe A positions relative to the stable BC core for TFIID (A), TFIID-SCP (B), TFIID-TFIIA (C), and TFIID-TFIIA-SCP samples (D). Inset in (A): Class averages corresponding to specific lobe A measurements from the TFIID sample.
See also Figure S1.
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5. Figure 3
TFIIA-Mediated Binding of SCP DNA to the Rearranged State of TFIID
(A and B) Cryo-EM 2D reference-free class averages of the rearranged conformation (A) and the canonical conformation (B) shown alongside contour models.
(C and D) 3D reconstructions of the rearranged conformation for TFIID-TFIIA-SCP at 32 Å (C) and TFIID at 35 Å (D). Lobe A is in yellow and the density attributed to DNA in (C) is shown in green.
(E and F) 3D reconstructions of the canonical conformation for TFIID-TFIIA-SCP at 32 Å (E) and TFIID at 35 Å (F). The stable BC core is colored blue for each model, and lobe A is in orange. Scale bar is 200 Å.
See also Figures S2, S3, and S7.
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6. Figure 4
Organization of Promoter DNA and TFIIA within the TFIID-TFIIA-SCP Complex
(A) Cryo-EM reconstruction of TFIID-TFIIA-SCP(−66) at 35 Å (left column) aligned with the cryo-EM reconstruction of TFIID-TFIIA-SCP (center) and their difference map (right). DNA density is colored in green, and lobe A and the BC core are colored in yellow and blue, respectively. Scale bar is 100 Å.
(B–D) 2D reference-free class averages for the rearranged TFIID-TFIIA-SCP complex with Nanogold labels on SCP DNA at +45 (B), SCP DNA at TATA (C), and TFIIA (D).
(E) 2D reference-free class average for the canonical state of TFIID-TFIIA-SCP containing Nanogold labeled on TFIIA. 3D models are shown alongside high-defocus averages (density threshold at σ = 3.5) with a gold sphere marking the localization of Nanogold for each experiment. See Extended Experimental Procedures for a detailed discussion about generating low-defocus class averages. Summary of gold labeling results for (B)–(D) are shown in (A), second row.
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7. Figure 5
Core Promoter Architecture Dictates TFIIA-Dependent and -Independent Interactions of TFIID with Core Promoter DNA
(A and B) DNase I (A) and MPE-Fe (B) footprinting of TFIID-SCP and TFIID-TFIIA-SCP.
(C) DNase I footprinting on wild-type and mutant SCP DNA sequences. 5′-labeled downstream probes were analyzed for DNase I protection in the presence or absence of TFIIA for wild-type, mutant TATA (mTATA), Inr (mInr), and MTE/DPE (mMTE/DPE). See also Figures S4, S5, and S6.
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8. Figure 6
Structural Description of the Rearranged TFIID-TFIIA-SCP Complex Relative to the TSS
Promoter DNA for SCP(−66) was docked into the TFIID-TFIIA-SCP(−66) map (shown in mesh).
(A and B) DNA models were colored based on protection patterns for DNase I and MPE-Fe. Red and pink surfaces indicate cleavage, the blue surface indicates partial cleavage, and lack of surface indicates protection. Asterisk (∗) in (B) indicates a DNase I hypersensitive site at +3. Black lines in (B) indicate regions of continuous protection along SCP helix.
(C) The proposed location of the crystal structures of TBP-TFIIA-TFIIB on TATA box DNA is in close proximity to Inr (Protein Data Bank ID codes 1VOL and 1NVP). The unresolved DNA path between Inr and TATA is indicated by a dotted line. See also Movie S2.
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9. Figure 7
Model of TFIID’s Interaction with Core Promoter DNA in a Conformation- and TFIIA-Dependent Fashion
(A–C) Conformations adopted by TFIID-TFIIA.
(D–F) Conformations adopted by TFIID alone.
(A and B) TFIIA stabilizes TFIID in the canonical conformation.
(C) The addition of SCP DNA stabilizes the rearranged conformation for the ternary complex TFIID-TFIIA-SCP.
(D and E) In contrast to TFIID-TFIIA (A and B), TFIID adopts a conformational landscape that populates canonical and rearranged states equally.
(F) Upon addition, SCP DNA is bound by TFIID in the rearranged conformation. Brackets ([ ]) denote that (F) was observed only through biochemical footprinting.
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10. Figure S1
Focused Classification and Positional Measurements of Lobe A within TFIID, Related to Figures 1 and 2
(A) Selected classums from 2D reference-free classification of all particles. The BC core is defined to be the rigid protein density that connects lobe B to lobe C.
(B) Strategy for alignment and classification. Particles were selected based upon membership within characteristic 2D reference-free averages that were calculated from all particles within a given data set. These selected particles were then aligned to the rigid BC core and classified within a mask centered on lobe A.
(C) Resulting subclassified classums from negatively stained TFIID particles after hierarchical ascendant classification.
(D) Resulting subclassified classums from cryo-EM of TFIID particles after hierarchical ascendant classification.
(E) Schematic for measuring lobe A position within subclassified classums. The position of lobe A was projected onto the B-C axis to provide a measurement for lobe A’s localization within TFIID.
(F) Histogram of lobe A positions for negatively stained (left) and vitrified TFIID (right).
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11. Figure S2
Orthogonal Tilt Reconstruction and Model Validation for Canonical and Rearranged Reconstructions Derived from TFIID-IIA-SCP Data, Related to Figure 3
(A) OTR tilt pairs. Scale bar, 200 nm.
(B) 2D reference-free class averages for −45° tilt data.
(C) Strategy for validating OTR models.
(D) Untilted TFIID-IIA-SCP 2D reference-free class averages that were used to assess the degree of arrangement and model quality for OTR class volumes.
(E) Highest and lowest resolution results from Fourier Ring Correlation (FRC) between model projections and class averages in (D).
(F) 3D model projections for refined and validated OTR models. Only 5 out of 8 rearranged models (c – g) and 1 out of 2 (a) canonical models are shown for space considerations. For comparison, the corresponding projection for a previously determined TFIID negative stain structure of the canonical state is shown (b).
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12. Figure S3
3D Reconstruction Information for Cryo-EM Models from TFIID-TFIIA-SCP and TFIID, Related to Figure 3
(A) Rearranged cryo-EM model for TFIID-TFIIA-SCP shown at a lower isosurface threshold than Figure 3C.
(B) 2D reference-free class averages aligned to projections of the canonical cryo-EM model (top) and rearranged model (bottom) from the TFIID-IIA-SCP data.
(C) Euler angle distribution for cryo-EM models from TFIID-IIA-SCP.
(D) FSC curves for models from TFIID-IIA-SCP.
(E) Rearranged cryo-EM model for TFIID shown at a lower isosurface threshold than Figure 3D. Note that the density (assigned to DNA) connects across the central channel only for the TFIID-TFIIA-SCP sample.
(F) 2D reference-free class averages aligned to best-matching projections of the canonical cryo-EM model (top) and rearranged model (bottom) from TFIID.
(G) Euler angle distribution for cryo-EM models from TFIID (H) FSC curves for models from TFIID.
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13. Figure S4
Line Profiles of Wild-Type SCP Footprinting Gels, Related to Figure 5
(A) Line profile traces from DNase I footprinting gels for 5′-label upstream. Bold basepair numbers indicate hypersensitive sites.
(B) Line profile traces from DNase I footprinting gels for 5′-label downstream. Bold basepair numbers indicate hypersensitive sites.
(C) Line profile traces from MPE-Fe footprinting gels for 5′- label upstream.
(D) Line profile traces from MPE-Fe footprinting gels for 5′-label downstream.
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14. Figure S5
Line Profiles for DNase I Footprinting on mTATA Promoter, Related to Figure 5
(A) Line profiles for TFIID (blue) and TFIID-TFIIA (red) on ‘wild-type’ SCP.
(B) Line profiles for TFIID (blue) and TFIID-TFIIA (red) on mTATA promoter.
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15. Figure S6
TFIID-TFIIA Interacts with SCP(mTATA) within the Rearranged Conformation, Related to Figure 5
(A) 2D reference-free class averages calculated from cryo-EM data of TFIID-TFIIA-SCP(mTATA) are shown alongside 2D reference-free class averages from TFIID-TFIIA-SCP.
(B) 3D models of the rearranged conformation for TFIID-TFIIA-SCP (see Figure 3C) and TFIID-TFIIA-SCP(mTATA) at 34Å.
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16. Figure S7
Immunoprecipitation of a Ternary TFIID-IIA-SCP Complex, Related to Figure 3
(A) Purification strategy.
(B) SDS-PAGE shows stoichiometric addition of TFIIA to purified complex. Note that the DNA did not stain within the gel.
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