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Methylation status of human chorionic gonadotropin beta subunit promoter and TFAP2A expression as factors regulating CGB gene expression in placenta 

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Presentation on theme: "Methylation status of human chorionic gonadotropin beta subunit promoter and TFAP2A expression as factors regulating CGB gene expression in placenta "— Presentation transcript:

1 Methylation status of human chorionic gonadotropin beta subunit promoter and TFAP2A expression as factors regulating CGB gene expression in placenta  Aleksandra Głodek, Ph.D., Marta J. Kubiczak, M.Sc., Grzegorz P. Walkowiak, M.Sc., Ewa Nowak-Markwitz, Anna Jankowska, Ph.D.  Fertility and Sterility  Volume 102, Issue 4, Pages e8 (October 2014) DOI: /j.fertnstert Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Relative expression level of (A) CGB1-9, (B) CGB3–9, and (C) CGB1–2 in chorionic (CT) and placental (PT) tissue. Fertility and Sterility  , e8DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Relative expression level of (A) SP1, (B) SP3, (C) SP1/SP3, and (D) TFAP2A in chorionic (CT) and placental (PT) tissue. Fertility and Sterility  , e8DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Methylation status of (A, B) CGB3–9 and (C, D) CGB1–2 promoter sequence in chorionic (CT) and placental (PT) tissue. Fertility and Sterility  , e8DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

5 Supplemental Figure 1 CGB genes promoter sequence alignment performed according to the ClustalW2 algorithm. Parallel arrows indicate AP2 and Sp1 binding sites, while the kinked arrow shows transcription start site; frames mark CpGs analyzed in MSP assays. Fertility and Sterility  , e8DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions


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