1. Ch. 19 Warm-up Why do many scientists classify viruses as non-living?
Draw the basic structure of a virus. Label and define capsid, viral envelope and nucleic acid.

2. Ch. 19 Warm-up
Draw the lytic/lysogenic cycle.
What stage of the lytic-lysogenic cycle is a virus virulent? Temperate?
What determines a host range?

3. Viruses
Chapter 19

4. What you must know: The components of a virus.
The differences between lytic and lysogenic cycles.

5. Bacteria vs. Viruses Bacteria Virus Prokaryotic cell
Most are free-living (some parasitic)
Relatively large size
Antibiotics used to kill bacteria
Not a living cell (genes packaged in protein shell)
Intracellular parasite
1/1000 size of bacteria
Vaccines used to prevent viral infection
Antiviral treatment

6. Viruses Very small (<ribosomes) Components = nucleic acid + capsid
Nucleic acid: DNA or RNA (double or single-stranded)
Capsid: protein shell
Some viruses also have viral envelopes that surround capsid
Limited host range (eg. human cold virus infects upper respiratory tract)
Reproduce within host cells

10. Simplified viral replicative cycle

11. Bacteriophage
Virus that infects bacterial cells

12. Video: t4 Phage infection

13. Lytic Cycle of T4 Phage

14. Bacteriophage Reproduction
Lytic Cycle:
Use host machinery to make copies of virus
Death of host cell by rupturing it (lysis)
Virulent phages replicate by this method
Lysogenic Cycle:
Phage DNA incorporated into host DNA and replicated along with it
Phage DNA = prophage
Temperate Phage: uses both methods of replication

15. Lytic Cycle vs. Lysogenic Cycle

16. Animal viruses have a membranous envelope
Host membrane forms around exiting virus
Difficult for host immune system to detect virus

17. Video: How Dengue Virus enters a cell

18. Retrovirus RNA virus that uses reverse transcriptase (RNA  DNA)
Newly made viral DNA inserted into chromosome of host
Host transcribes viral DNA (= provirus) to make new virus parts
Example: HIV (Human Immunodeficiency Virus)

19. HIV = Retrovirus

20. HIV Infects white blood cells HIV+: provirus (DNA inserted)
AIDS: active viral reproduction

21. Video: HIV Life Cycle

22. Other Human Viruses Herpes virus Smallpox
Herpes Simplex Virus 1 (HSV-1)
Herpes Simplex Virus 2 (HSV-2)
Eradicated in 1979 due to worldwide vaccination campaigns

23. Ebola Transmission: contact with contaminated blood or bodily fluids
Symptoms: fatigue, fever, severe headache, vomiting, diarrhea, rash, bleeding
May appear 2-21 days after exposure

24. Ebola Treatment: Intravenous fluids, balance electrolytes
Experimental: antiviral drugs, plasma transfusions from survivors, antibodies (Zmapp)
No vaccine (yet)
Statistics:
2014 Ebola Outbreak (worldwide): 21,382 cases, deaths (*as of 1/19/15)
Seasonal Influenza: estimated 36,000 deaths in U.S. each year (2015: mutated H3N2 strain)

25. 2014 Ebola Outbreak

26. Cumulative Ebola Cases

27. Vaccines
Weakened virus or part of pathogen that triggers immune system response

28. Emerging viruses = mutation of existing viruses

29. Viroids Small, circular RNA molecules that infect plants
Cause errors in regulatory systems that control plant growth
Eg. coconut palms in Philippines

30. Prions
Misfolded, infectious proteins that cause misfolding of normal proteins
Eg. mad cow disease (BSE),Creutzfeldt-Jakob disease (humans), scrapie (sheep)

31. Diseases caused by prions
Prions act slowly – incubation period of at least 10 years before symptoms develop
Prions are virtually indestructible (cannot be denatured by heating)
No known cure for prion diseases