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IVM and gene expression of sheep cumulus–oocyte complexes following different methods of vitrification  Bita Ebrahimi, Mojtaba Rezazadeh Valojerdi, Poopak.

Published bySévérine Géraldine Laviolette Modified over 6 years ago

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Presentation on theme: "IVM and gene expression of sheep cumulus–oocyte complexes following different methods of vitrification  Bita Ebrahimi, Mojtaba Rezazadeh Valojerdi, Poopak."— Presentation transcript:

1 IVM and gene expression of sheep cumulus–oocyte complexes following different methods of vitrification  Bita Ebrahimi, Mojtaba Rezazadeh Valojerdi, Poopak Eftekhari-Yazdi, Hossein Baharvand, Ali Farrokhi  Reproductive BioMedicine Online  Volume 20, Issue 1, Pages (January 2010) DOI: /j.rbmo Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

2 Figure 1 (A and B) Vitrified–warmed degenerate COC observed with stereomicroscope (A) and stained with calcein-AM and ethidium homodimer (B) ×20. (C and D) Vitrified–warmed healthy COC observed with stereomicroscope (C) and stained with calcein-AM and ethidium homodimer (D) ×20. (E and F) Percentage of healthy vitrified–warmed COC before and after in-vitro maturation, respectively, in all experimental groups. Cont, control; ConV, conventional straw vitrification; CTV, cryotop vitrification; SSV, solid-surface vitrification. Data are mean±SE. a versus b and c versus d indicate significant differences (P<0.05). Reproductive BioMedicine Online  , 26-34DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

3 Figure 2 Different nuclear stages of oocytes after in-vitro maturation: (A) germinal vesicle (×40); (B) germinal vesicle break down (×40); (C) mature oocyte (metaphase II) (×40); (D) percentage of different nuclear stages of oocytes after in-vitro maturation in vitrification and control groups. Cont, control; ConV, conventional straw vitrification; CTV, cryotop vitrification; SSV, solid-surface vitrification. Data are mean±SE. a versus b, c versus d and e versus f indicate significant differences (P<0.05). Reproductive BioMedicine Online  , 26-34DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

4 Figure 3 Fold differences of relative maturation gene expression compared by analysis of variance and Tukey’s test in vitrification and control groups. Cont, Control; ConV, conventional straw vitrification; CTV, cryotop vitrification; SSV, solid-surface vitrification. Data are mean±SEM. a=P⩽0.05; b=P⩽0.01; c=P⩽0.008; d=P⩽0.001. Reproductive BioMedicine Online  , 26-34DOI: ( /j.rbmo ) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

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