1. Cryo-EM structure of I27[L = 35] RNCs
Cryo-EM structure of I27[L = 35] RNCs. (A) Cryo-EM reconstruction of the I27–TnaC[L = 35] RNC. The ribosomal small subunit is shown in yellow, the large subunit in gray, the peptidyl-tRNA with the nascent chain in green, and an additional density ...
Cryo-EM structure of I27[L = 35] RNCs. (A) Cryo-EM reconstruction of the I27–TnaC[L = 35] RNC. The ribosomal small subunit is shown in yellow, the large subunit in gray, the peptidyl-tRNA with the nascent chain in green, and an additional density corresponding to I27 at the ribosome tunnel exit in red. The black cartoon eye and dashed lines indicate the angle of view in C. The density contour level for feature visualization is at 1.7 times rmsd. (B) Rigid-body fit of the I27 domain (PDB ID code 1TIT) to the cryo-EM density map displaying from high (Left) to low (Right) contour levels at 2.6, 2.0, and 1.4 rmsd, respectively. N and C represent the N and C termini of the I27 domain, respectively. (C) View looking into the exit tunnel (arrow) with density for the nascent chain (nc) in dark green. Ribosomal proteins uL29 (blue; PDB ID code 4UY8), uL24 (light green; the β hairpin close to I27 domain was remodeled based on PDB ID code 5NWY) and the fitted I27 domain (red) are shown in cartoon mode; 23S RNA and proteins not contacting I27 are shown as density only. The density contour level is at 5 rmsd excluding tRNA, nascent chain, and I27 domain, which are displayed at 1.7 rmsd.
Pengfei Tian et al. PNAS 2018;115:48:E11284-E11293
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