Presentation is loading. Please wait.

Presentation is loading. Please wait.

Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a*/* ESC clones (B) were injected into C57BL/6N blastocysts.

Published byHubert Fields Modified over 6 years ago

Similar presentations

Presentation on theme: "Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a*/* ESC clones (B) were injected into C57BL/6N blastocysts."— Presentation transcript:

1 Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a*/* ESC clones (B) were injected into C57BL/6N blastocysts and scored for their chimeric contribution. Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a*/* ESC clones (B) were injected into C57BL/6N blastocysts and scored for their chimeric contribution. All ESC clones gave reasonable numbers of chimeric animals relative to the implanted embryos (supplementary Table S1) and, as expected, most of the chimeras were males as we exclusively used male ESC clones. ■ male, female, n.d. C, DComparison between chimeric contribution estimated on basis of coat‐color versus genetic chimerism, tested in various tissues. Southern blot analysis was performed with a probe that distinguishes between a wild‐type Trp53 allele or the floxed Trp53 allele reflecting the contribution by the host ESCs or cultured ESCs, respectively (example in supplementary Fig S3). Controls are wild‐type spleen (0% chimerism expected) and F1 offspring of chimeras (50% chimerism expected). (C) Genetic chimerism of Rb1F/F;Trp53F/F chimeras with coat color chimerism ranging from 70 to 100% (average 84%, n = 7). (D) Genetic chimerism of Nf2F/F;Trp53F/F;Cdkn2a*/* chimeras with coat color chimerism ranging from 85 to 100% (average 95%, n = 4). ESurvival curves of Rb1F/F;Trp53F/F mice intratracheally injected with Ad5‐Cre. Black line, conventional mice; red line, chimeras. FSurvival curves of Nf2F/F;Trp53F/F;Cdkn2a*/* mice intrathoracically injected with Ad5‐Cre. Black line: conventional mice; Red line: chimeras. GTypical example of a neuroendocrine carcinoma (Small Cell Lung Cancer) in the lung (left panel) and a metastatic lesion in the liver (right panel). HTypical example of a mesotheliomatous lesion in the thoracic cavity. Tumor cells are either spindle sarcomatoid cells (left panel) or vacuolated epithelioid cells (right panel). Ivo J Huijbers et al. EMBO Mol Med. 2014;6: © as stated in the article, figure or figure legend

Download ppt "Validation of chimeras A, BThree Rb1F/F;Trp53F/F ESC clones (A) and two Nf2F/F;Trp53F/F;Cdkn2a*/* ESC clones (B) were injected into C57BL/6N blastocysts."

Similar presentations

DNA Chips Attach DNA to tiny spots on glass slides (i.e., chip). Hybridize fluorescently-labeled DNA probes to chip. Detect hybridization to different.

DNA Chips Attach DNA to tiny spots on glass slides (i.e., chip). Hybridize fluorescently-labeled DNA probes to chip. Detect hybridization to different.
H. influenzae genes differentially regulated during growth in complex media and during lung infection in the presence of corticosteroids AVenn diagram.

H. influenzae genes differentially regulated during growth in complex media and during lung infection in the presence of corticosteroids AVenn diagram.
Hyp mice show hypertension and increased NCC expression and phosphorylation A–ESerum intact Fgf23 concentration (A, n = 8–9, Student's t‐test, *P = 0.0001.

Hyp mice show hypertension and increased NCC expression and phosphorylation A–ESerum intact Fgf23 concentration (A, n = 8–9, Student's t‐test, *P = 
Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD 

Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD 
TZD enhances cisplatin sensitivity through suppression of endotrophin‐mediated EMT, fibrosis and angiogenesis.A,B.Schematic diagram for allografts (A),

TZD enhances cisplatin sensitivity through suppression of endotrophin‐mediated EMT, fibrosis and angiogenesis.A,B.Schematic diagram for allografts (A),
GLUT3 is a direct target of TCF4/β‐catenin

GLUT3 is a direct target of TCF4/β‐catenin
Volume 86, Issue 1, Pages (July 1996)

Volume 86, Issue 1, Pages (July 1996)
Heatmaps of the gene mutation distributions in oncogene‐signaling blocks for six representative cancer types. Heatmaps of the gene mutation distributions.

Heatmaps of the gene mutation distributions in oncogene‐signaling blocks for six representative cancer types. Heatmaps of the gene mutation distributions.
The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.

The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.
Globin Gene Expression Is Reprogrammed in Chimeras Generated by Injecting Adult Hematopoietic Stem Cells into Mouse Blastocysts  Hartmut Geiger, Stefanie.

Globin Gene Expression Is Reprogrammed in Chimeras Generated by Injecting Adult Hematopoietic Stem Cells into Mouse Blastocysts  Hartmut Geiger, Stefanie.
Relationship between Genotype and Phenotype

Relationship between Genotype and Phenotype
Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD 

Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD 
AAV8‐hAAT‐FGF21 treatment improves liver fibrosis

AAV8‐hAAT‐FGF21 treatment improves liver fibrosis
The absence of COL6 in PyMT mice sensitizes tumours to cisplatin treatment.A.Eleven week old FP635/PyMT and FP635/PyMT/COL6−/− mice were given cisplatin.

The absence of COL6 in PyMT mice sensitizes tumours to cisplatin treatment.A.Eleven week old FP635/PyMT and FP635/PyMT/COL6−/− mice were given cisplatin.
Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.

Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.
CAR T cell dose and percentages of CAR‐positive cells within CAR T cell products CAR T cell dose and percentages of CAR‐positive cells within CAR T cell.

CAR T cell dose and percentages of CAR‐positive cells within CAR T cell products CAR T cell dose and percentages of CAR‐positive cells within CAR T cell.
by Hiromi Gunshin, Carolyn N. Starr, Cristina DiRenzo, Mark D

by Hiromi Gunshin, Carolyn N. Starr, Cristina DiRenzo, Mark D
Metabolic profile of Tfe3 KO mice

Metabolic profile of Tfe3 KO mice
Both haematopoietic and non‐haematopoietic compartments are involved in myocarditis development.A.Kaplan‐Meier survival curves showing survival of bone.

Both haematopoietic and non‐haematopoietic compartments are involved in myocarditis development.A.Kaplan‐Meier survival curves showing survival of bone.
Volume 29, Issue 1, Pages (April 2014)

Volume 29, Issue 1, Pages (April 2014)

Similar presentations

Ads by Google