1. Matrix Mineralization
Supplemental Data 3 a
mRNA level
(Fold change)
Runx2
OSX
COLL1
ALP
0.2
0.4
0.6
0.8
1.0
1.2 *
SaOS2
Runx2
OSX
COLL1
ALP
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
mRNA level
(Fold change) *
U2OS b
ALP Activity
(Fold Change)
0.2
0.4
0.6
0.8
1.0
1.2
1.4
Day 1
Day 2
Day 3
Day 4 *
SaOS2
Day 1
Day 2
Day 3
Day 4
ALP Activity
(Fold Change) *
0.2
0.4
0.6
0.8
1.0
1.2
1.4
U2OS c d e
ALP Activity
SaOS2
U2OS
Parental
shMT2A
Matrix Mineralization
SaOS2
U2OS
Parental
sh MT2A
0.2
0.4
0.6
0.8
1.0
1.2
SaOS2
U2OS
Mineral content (A.U.) *