1. Volume 4, Issue 2, Pages 113-120 (June 1997)
Stimulation and inhibition of androgen-mediated proliferation in human prostate tumor cell line LNCaP: biological and pathophysiological impact 
Hideaki Hoshino, Kou Sakabe, Yoji Katsuoka, Kanji Seiki, Nobuo Kawamura 
Pathophysiology 
Volume 4, Issue 2, Pages (June 1997)
DOI: /S (97)

2. Fig. 1
Cell proliferation yields obtained when LNCaP cells were grown in 10% CDHuS. The serum was supplemented with: (a) 3×10−12–3×10−7 mol/l DHT; (b) 3×10−12–3×10−7 mol/l DHT plus 3×10−8 mol/l FLU (–∘–) or 30 μg SPN (-▴-). Values are means±S.D. of six determinations. *P<0.01 vs. control value (no DHT).
Pathophysiology 1997 4, DOI: ( /S (97) )

3. Fig. 2
Effect of serum concentration on cell proliferation when LNCaP cells were grown in medium supplemented with 1 to 30% CDHuS alone (□) or with 3×10−10 mol/l DHT (■). Values are means±S.D. of six determinations. *P<0.01 vs. value of serum alone.
Pathophysiology 1997 4, DOI: ( /S (97) )

4. Fig. 3
The kinase assay samples from LNCaP cells were measured by the non-radioisotopic PKC assay system. The color bands on the gel were analyzed in a scanning densitometer at 570 nm and expressed as percent activity. The serum was supplemented with: (a) 3×10−12–3×10−7 mol/l DHT; (b) 3×10−12–3×10−7 mol/l DHT plus 3×10−8 mol/l FLU (–∘–) or 30 μg SPN (-▴-). Values are means±S.D. of six determinations. *P<0.01 vs. control value (no DHT).
Pathophysiology 1997 4, DOI: ( /S (97) )

5. Fig. 4
DNA synthetic activity of isolated liver cell nuclei incubated with the cytoplasmic extract from the LNCaP cells exposed to: (a) 3×10−12–3×10−7 mol/l DHT; (b) 3×10−12–3×10−7 mol/l DHT plus 3×10−8 mol/l FLU (–∘–) or 30 μg SPN (-▴-). Values are means±S.D. of six determinations. *P<0.01 vs. control value (no DHT).
Pathophysiology 1997 4, DOI: ( /S (97) )

6. Fig. 5
Immunoblot of the extract from LNCaP cells treated with 3×10−12–3×10−7 mol/l DHT. Peptide bands containing cdc2 protein molecules were visualized by alkaline phosphatase-staining.
Pathophysiology 1997 4, DOI: ( /S (97) )

7. Fig. 6
Immunohistochemical staining of androgen receptors (AR) in LNCaP cells. Specific AR staining in cells was confined exclusively to the nucleus, with none present in the extranuclear compartment. Magnification: X360
Pathophysiology 1997 4, DOI: ( /S (97) )