1. DXS (exp. level: 1632) DXR (exp. level: 737) MCT (exp. level: 402)
CMK (exp. level: 251)
Chlorophylls m=0.025; r=0.11; P=0.456
Chlorophylls m=0.006; r=0.31; P=0.050
Chlorophylls m=5x10-5; r=0.01; P=0.929
Chlorophylls m=3x10-4; r=0.08; P=0.609
Carotenoids m=-0.061; r=0.29; P=0.055
Carotenoids m=0.006; r=0.37; P=0.016
Carotenoids m=5x10-4; r=0.11; P=0.491
Carotenoids m=2x10-5; r=0.004; P=0.979
Sterols m=-0.013; r=0.12; P=0.446
Sterols m=0.004; r=0.25; P=0.104
Sterols m=-5x10-4; r=0.12; P=0.456
Sterols m=0.001; r=0.32; P=0.032
MDS (exp. level: 604)
HDS (exp. level: 1011)
HDR (exp. level: 975)
Chlorophylls m=0.011; r=0.37; P=0.019
Chlorophylls m=0.002; r=0.05; P=0.738
Chlorophylls m=-0.008; r=0.07; P=0.691
Carotenoids m=0.012; r=0.40; P=0.012
Carotenoids m=-0.002; r=0.03; P=0.834
Carotenoids m=-0.004; r=0.02; P=0.900
Sterols m=-8x10-5; r=0.0004; P=0.981
Sterols m=0.002; r=0.04 P=0.819
Sterols m=-0.011; r=0.16 P=0.384
Suplemental Figure S3. Gene-to-metabolite correlation analysis identifies genes with highest impact on chlorophyll and carotenoid accumulation. The expression levels of genes involved in the plastidial MEP pathway were modulated in transgenic Arabidopsis lines (fold-change vs. untransformed controls on x-axis). Major end products derived from this pathway (chlorophylls and carotenoids) were quantified (fold-change vs. untransformed controls on y-axis). Important parameters of the statistical analysis (m = slope; r = Pearson correlation coefficient; P = Student’s t-test P-value) and expression levels of each gene in rosette leaves (based on AtGenExpress microarray data resource (Schmid et al., 2005)) are provided above the graphs. Gray background is used to indicate positive gene-to-metabolite correlations. Acronyms: DXS, 1-deoxy-D-xylulose 5-phosphate synthase; DXR, 1-deoxy-D-xylulose 5-phosphate reductoisomerase; MCT, 2C-methyl-D-erythritol 4-phosphate cytidyltransferase; CMK, 4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol kinase; MDS, 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; HDS, 1-hydroxy-2-methyl-2-butenyl 4-phosphate synthase; HDR, 1-hydroxy-2-methyl-2-butenyl 4-phosphate reductase.