1. 1.5 The Origin of Cells Cells come from pre-existing cells
The first cells arose from non-living materials
Endosymbiosis

2. Cell Division & The Origin of Cells
All cells are formed by the division of pre-existing cells.
You are made of trillions of cells.
Any cell that is produced by your body is the product of cell division from another cell.
You originally started life as a single cell, a zygote, that underwent numerous cell divisions to produce the trillions of cells that comprise you today.

3. Even that single celled zygote came from other cells – the combination your father’s sperm cell and your mother’s egg cell.
We can trace the origins of all the cells in our parents back to the zygotes from which they developed, to our human ancestors before them, to human’s pre-existing ancestral species, all the way back to the earliest cells on Earth.

4. We, and all other living things on this planet, are descendants from the “first cell”.
But if all cells come from pre-existing cells, where did the first cell come from?
How did life start on this planet?

5. Problems for starting life on Earth
How could the lifeless ball of rock that the planet Earth was 3.5 billion years ago, become home to such lush vegetation and a wide variety of bacteria, fungi, protists, and animals that we see today?
There are 4 problems which needed to be overcome for life on Earth to exist.

6. Requirements to start life
Production of Simple Organic Compounds
The assembly of organic compounds into polymers
Development of a mechanism for inheritance.
Formation of membranes

7. 1. Production of Simple Organic Compounds
Life as we know it is based on organic compounds (compounds containing carbon and hydrogen), such as amino acids (the building blocks of proteins)
But early Earth only had inorganic matter: rocks, minerals, gases, water….

8. Abiogenesis had to occur.
abiogenesis: the creation of organic matter from inorganic matter)
It is believed that organic molecules were formed in the shallow waters of the oceans as the products of chemical reactions between compounds in the atmosphere and the water.

9. Miller and Urey
Scientists Stanley Miller and Harold Urey performed a ground breaking experiment in 1953
They recreated the conditions of early Earth and proved that organic compounds could be synthesized from inorganic compounds.

10. N2 = nitrogen gas H2O = water
H2 = hydrogen gas NH3 = ammonia gas
CO2 = carbon dioxide gas * CH4 = methane gas

11. The Experimental Design
The apparatus included an “oceanic compartment” and an “atmospheric compartment”
The H2O in the oceanic compartment was heated to evaporate and cooled to condense – thereby recreating the H2O cycle.
Since early Earth did not have an ozone layer, they kept the system at a warm temperature and exposed it to UV radiation
Generated electric sparks to simulate lightning

13. The Results After 1 week:
15% of the carbon was now found in organic form!
13 of the 20 amino acids had formed inside the primordial soup!
Sugars had formed!
The nitrogenous base adenine (a component of DNA and ATP) had formed!!!

14. The Conclusion
Early Earth had the proper conditions for abiogenesis.

15. 2. Assembly of these molecules into polymers
Organisms are organized!
Simple organic molecules would have needed to undergo a process of polymerization to form the larger more complex organic chemicals required by cells.

16. Deep-Sea Vents
Organic molecules could have first formed around hydrothermal vents – places where hot water emanated from beneath the ocean floor.
Form when cracks in the crust of the seabed expose sea water to rocks below which are heated by magma

17. As the hot water rises it picks up countless minerals along the way.
Hydrothermal vents are sometimes referred to as black smokers because the water coming out of them contains so many dark minerals it looks like smoke.
The chemicals and source of energy in this environment could be suitable for the formation of biological polymers.

18. 3. The development of a mechanism for inheritance
Today, most organisms use DNA as its molecule for heredity.
To replicate DNA and pass it on to the next generation, enzymes are required
However, enzymes cannot be made without DNA.
Therefore, it is unlikely that DNA was the early molecule for heredity

19. Ribozymes
However, the small sequences of the molecule RNA can act as enzymes and replicate itself.
These are called ribozymes
Thus, RNA may be the early molecule for hereditary.

20. 4. Formation of Membranes
Water is important to life but tends to depolymerize (break down)molecules
Many compounds dissolve in water, making it difficult to organize into polymers
The formation of closed membranes is likely an early and important event in the origin of cellular life
It allows for the development of an internal chemistry different from the external environment

21. Coacervates
Coacervate - a microscopic sphere that forms from lipids in water.
Forms spontaneously due to the hydrophobic forces between the water and lipid molecules.
Can maintain an internal chemical environment different from the surrounding environments.
Coacervates can be selectively permeable

22. Coacervates
Coacervates (lipids)

23. Although they are not living organisms, coacervates are a significant step toward the formation of cells.
They solve the problem of protecting polymers from their destructive environments.
Could be primitive versions of the first cell membranes

24. (remember our cell membranes are lipid based)
PROTOBIONTS – the first precursors to cells, were likely coacervate droplets which included polynucleotides (DNA or RNA)
(remember our cell membranes are lipid based)
Overtime, true cell membranes evolved and other characteristics of cells developed.
Cellular respiration
Asexual reproduction

25. Spontaneous Generation
While spontaneous generation must of occurred billions of years ago to give rise to the first cells, spontaneous generation of cells and organisms does not now occur on Earth.
This is supported by experiments conducted by Louis Pasteur in 1864.

26. Spontaneous Generation
Before Pasteur, many people believed spontaneous generation possible and believed it explained why mould would grow seemingly out of nowhere, and why fruit flies would appear in a room that didn’t have any previously.
Many people believed that spontaneous generation could occur as long as there was access to air.

27. Pasteur’s Experiments
He created a nutrient broth by boiling water containing yeast and sugar, thereby killing the organisms
When the broth was kept in a sealed flask it remained unchanged – no fungi or organisms appeared.

28. When exposed to air that travelled through a swan neck, it also remained unchanged because the apparatus prevented microorganisms from entering the broth
When the swan neck was removed, microorganisms could enter the broth and proliferate

31. Where did all the oxygen come from?
1/5 of the air you are breathing right now is oxygen.
However, there was none at all present 4 billion years ago.
The earliest life forms on Earth were bacteria and they lived in an environment with an atmosphere of mostly CO2
Thus, early life forms were anaerobic cells (did not require oxygen)

32. These single-celled organisms would consume organic molecules (i. e
These single-celled organisms would consume organic molecules (i.e. simple sugars) that were forming from chemical reactions on Earth
The more they reproduced, the more food that was consumed.

33. After million of years, their population would have reached such large numbers that food began to be scarce.
In this food shortage, bacteria that could make their own food would have an advantage.

34. ~3.5 billion years ago, bacteria (that is believed to be related to today’s cyanobacteria)developed the ability to photosynthesize.
Must have contained a form of chlorophyll

35. Gives bacteria a source of energy (sunlight) to survive
Development of photosynthesis was one of the most significant evens in the history of Earth
Gives bacteria a source of energy (sunlight) to survive
Created a mass pollution of the atmosphere
Pollution of oxygen!!!

36. Oxygen gas is toxic to the kinds of bacteria which preceded photosynthetic ones, so this pollution would have eventually killed off large populations of anaerobes.
Anaerobic bacteria that survived would live in mud of places protected from the new oxygen-rich atmosphere.

37. The ability of an organism to make its own food gives it a distinct advantage over those that cannot.
As a result, photosynthetic bacteria proliferated and produced more and more oxygen

38. Endosymbiosis
3.8-2 bya, bacteria (prokaryotic cells) were the only organisms on Earth
The first fossils of cells with a nucleus (eukaryotes) is from around 2 bya.
How did prokaryotes develop into eukaryotes?
Endosymbiosis is the most popular theory

39. Endosymbiosis
ENDOSYMBIOSIS: when one organism lives within the other and they both benefit
Ex: bacteria that live inside our digestive tract

40. Endosymbiotic Theory
The chloroplasts and mitochondria that are found inside eukaryotic cells today were once independent prokaryotic cells.
They were engulfed by a bigger prokaryotic cell.
Rather than being digested, the prokaryotes were kept alive inside the host cell in exchange for their services

41. Endosymbiotic Theory
The host cell would provide protection for the smaller prokaryotic cell
The engulfed cell would be beneficial to the host if
it was photosynthetic like the chloroplast (providing food) for the host or
able to metabolize food efficiently and produce energy for the host like the mitochondrion

43. Evidence for Endosymbiosis
Mitochondria and chloroplasts are very similar to prokaryotic cells. The many similarities suggest that they were once independent prokaryotic cells.

44. Evidence of Endosymbiosis
Chloroplasts and mitochondria are surrounded by a double membrane (like the prokaryotic plasma membranes)
Mitochondria and bacteria (a prokaryote) have a similar size

45. Evidence of Endosymbiosis
Mitochondrial and bacterial ribosomes are very similar in size and shape.
Mitochondria and chloroplasts have their own DNA – which is circular like bacteria.
Mitochondria divide in a process similar to binary fission like bacteria

46. Problems with Endosymbiosis
The ability to engulf another cell and have it survive in the cytoplasm does not guarantee that the host cell can pass it on to its offspring the genetic code to synthesize the newly acquired organelle
When chloroplasts or mitochondria are removed from a cell, they cannot survive on their own.