1. Introduction to Gel Electrophoresis

2. Outline How to prepare a gel How to micropipet
Practice setting up electrophoresis
Discussion viewing of gel
In a later lab you will view and photograph your results

3. Agarose is weighed out

4. Agarose is diluted and boiled in buffer solution

5. Agarose solution is poured into gel holder
This is a “comb”

6. Notice the “sample wells”
Agarose cools and solidifies
Notice the “sample wells”
comb

7. Next: How to pipet into the sample well

9. Select either a 100 or 200 ul micropipet from your lab station
(1000 ul= 1 ml)
Set at 25 ul
Place on a yellow tip

10. Push plunger to “first stop”
Place tip in solution
Aspirate sample by releasing plunger

11. Carefully place the tip of the micropipet just inside the well
Dispense solution by pushing to second stop
Release tip by “ejection button”

12. Gel and samples are subjected to electric current

13. Samples migrate through the gel at different rates
Negative electrode
Positive electrode

14. View your DNA samples with UV light
Bio 22

15. Photograph the results