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Gaussian Chain Entropy only. Infinite extensibility. 2. Freely Jointed Chain. Entropy. Finite extensibility. Rigid rods. 3. Wormlike Chain. Entropy. Finite extensibility. Bending elasticity. Smooth curve like cooked spaghetti.
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Persistence length = 50nm = 6mm = bigger than a cell = 17m
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Mechanism of DNA virus assembly and DNA packaging
Rao and Feiss – Annu Rev Virol (2015) 2(1): 351–378 Costs of DNA packaging: DNA bending + Electrostatic repulsion of charged DNA + Entropic cost of confinement
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Measuring the force generated by T4 DNA packaging motors
Fuller et al PNAS (2007) 104: Tension in the DNA increases as packaging proceeds. Eventually the tether breaks. Speed of motpor decreases as tension increases.
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Differential Scanning Calorimetry of Bacteriophage .
Exothermic peaks associated with Capsid denaturation and DNA melting. Endothermic peak due to DNA ejection. Shows that there was significant stored energy in the packaged DNA Bauer et al J Virol (2015) 89:
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Kunze and Netz (2000)
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Brower-Toland et al 2002
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Mihardja et al (2006)
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Kulic and Schiessel 2004
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Tomschik et al 2005
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