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Increased blood levels of NKG2D+CD4+ T cells in patients with alopecia areata
Yong Hyun Jang, MD, PhD, Jin Kyeong Choi, PhD, Yun Hwan Jang, MD, Sun Young Moon, MD, Weon Ju Lee, MD, PhD, Seok-Jong Lee, MD, PhD, Young-Ae Choi, PhD, Sang-Hyun Kim, PhD, Do Won Kim, MD, PhD Journal of the American Academy of Dermatology Volume 76, Issue 1, Pages (January 2017) DOI: /j.jaad Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions
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Fig 1 Results of fluorescence-activated cell sorting (FACS) analysis of peripheral blood and natural killer group 2D (NKG2D)+CD4+ T, NKG2D+CD8+ T, and NKG2D+CD56+ NK cells in control and alopecia areata (AA) patient groups. Analysis of FACS data was carried out dividing into 4 times because of a large number of samples. The left bar graph indicates the average fold change of NKG2D+ cells from the 4 experiments (26 healthy controls and 43 patients with AA). NKG2D+ expressed on CD4+ T cells was significantly higher in AA patient groups compared to control groups, as with NKG2D+CD8+ T cells and NKG2D+CD56+ NK cells. The right dot plot shows the representative results taken from the third experiment (10 healthy controls and 10 patients with AA). Journal of the American Academy of Dermatology , DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions
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Fig 2 Double-immunofluorescence analysis with anti–natural killer group 2D (NKG2D; green) and anti-CD4 (red) antibodies. The merged image clearly shows infiltration of NKG2D+CD4+ T cells in the lesional hair follicles of patients with alopecia areata. The two white arrows indicate the location of hair follicle. Journal of the American Academy of Dermatology , DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions
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