1. Introduction of Biotechnology
Pre-AP Biology

2. Bellwork What does Biotechnology mean to you?
Do you know of any products that were produced by Biotechnology?

3. Objectives Define Biotechnology Define restriction enzyme
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Objectives
Define Biotechnology
Define restriction enzyme
Explain how restriction enzymes work

4. Terminology Restriction enzyme Restriction site Palindrome sequence
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Terminology
Restriction enzyme
Restriction site
Palindrome sequence
Sticky end
Blunt end
Restriction fragments

5. Definition of Biotechnology
The study and manipulation of living things for the purpose of making products that benefit society

6. PROPERTY OF PIMA COUNTY JTED, 2010
Think-Pair-Share
On the following slide you will watch a video describing advances in Biotechnology. While you are watching the video, write down some key products produced by this industry. Be ready to share
3. Think-pair-share
teacher presents a question
teacher gives wait time for student to form answer
teacher instructs students to share their answer with a partner
teacher calls on non-volunteers to share with the class
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7. Biotechnology Video
Biotechnology: A New Frontier

8. Significance of Basic Molecular Biology
Discovery of DNA as the genetic code
Understanding the processes involved in going from DNARNAProtein
Allowed us to develop molecular tools for manipulating DNA for advancements in
medical science
Agriculture
food industry

9. As Biotechnology developed, several molecular tools were created
Two main tools are
Restriction enzymes
Agarose gel electrophoresis

10. Introductory Activity
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Introductory Activity
Read excerpt and underline the letters to every time it appears in the text.
Draw a vertical line after the t.
The vertical line represents a cut site.
If you were to cut at that cut site with scissors, how many text fragments would you have? Write the number in the designated spot on the note-taker.

11. Restriction Enzymes Cut at specific sites along the DNA
Each enzyme recognizes a unique cut site
Reflect: How are restriction enzymes like the scissors in the introductory activity?
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12. Where are restriction enzymes found in nature?
Proteins found in bacteria
Protect bacteria against DNA from invading organisms including viruses or other bacteria
Protect the bacteria by cutting the foreign DNA
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13. Characteristics of the Restriction Site
Four to six nucleotides in length
Symmetrical
meaning the sequence for both strands are the same when read in 5’ to 3’ direction
Palindromic Sequence
Due to symmetry 3’ 5’ 3’ 5’
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14. Two Types of Cut Sites Blunt End
restriction enzyme cuts the two strands of DNA such that the ends are even across both strands
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15. Two types of Cut Sites Sticky Ends
restriction enzyme cuts the two strands in a staggered way
This results in one end of DNA being single-stranded and overhangs the other strand
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16. PROPERTY OF PIMA COUNTY JTED, 2010
Paraphrase
Identify at least two key points in your notes about: restriction site
Summarize your key points in your own words!
6. Paraphrase
student writes down one or two main ideas directly from the text or notes
student now paraphrases these ideas in their own words
teacher provides feedback
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17. Restriction Fragment DNA after cut with restriction enzymes
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Restriction Fragment
DNA after cut with restriction enzymes
Fragments created differ in size depending on where the restriction site is located
The size of the fragment is defined by the number of paired nucleotides along the fragment

18. Determination of Fragment Size
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Determination of Fragment Size
Can be determined by conducting agarose gel electrophoresis
Compare the DNA Fragments to known standards after they migrate in the gel under an electrical current and are stained.
What is the size of fragment in lane 2?

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20 Word Summary 48 37 18 21 14 13 23
In 20 words or less, summarize the most important aspects from today's lesson.
Word Summary
in 12 words or less, summarize the most important aspects from today's lesson
teacher uses a strategy to check all
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