1. Volume 114, Issue 2, Pages 324-328 (February 1998)
Two cytochromes P450 are major hepatocellular autoantigens in autoimmune polyglandular syndrome type 1 
Maria Grazia Clemente, Antonella Meloni, Petra Obermayer-Straub, Fulvia Frau, Michael Peter Manns, Stefano de Virgiliis 
Gastroenterology 
Volume 114, Issue 2, Pages (February 1998)
DOI: /S (98)
Copyright © 1998 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Pedigrees of 11 patients with APS-1. Circles, female subjects; squares, male subjects; slash across symbol, deceased subjects; gray symbols, APS-1 patients without liver disease; black symbols, APS-1 patients with liver disease. The numbers from 1 to 11 below the symbols correspond to the patient numbers in Table 1.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Indirect immunofluorescent photomicrograph showing the staining pattern of LKM autoantibodies in parallel sections of rat (A) liver and (B) kidney using serum from patient 10 at 1:100 dilution.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Western blotting of microsomal preparations from human liver. Lanes 1–11, patients with APS-1 (lane numbers correspond to patient numbers in Table 1 and Figure 1); lane 12, AIH type 2, LKM-1 positive (the target antigen of LKM-1 antibodies is CYP2D6, a 50-kilodalton protein; the protein bands at different molecular weight are still unidentified); lane 13, hepatitis C, LKM-1 positive; lane 14, AIH type 1, anti–smooth muscle antibody positive; lane 15, primary biliary cirrhosis, antimitochondrial positive (the band at approximately 70 kilodaltons indicates reactivity of the mitochondrial-positive serum with the E2 subunit of the mitochondrial pyruvate dehydrogenase complex, contaminating the microsomal subcellular protein fractions); lanes 16–18, insulin-dependent diabetes mellitus and celiac disease; lanes 19 and 20, Hashimoto's thyroiditis; lane 21, systemic lupus erythematosus. Markers on the left indicate the molecular weights in kilodaltons.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Western blotting performed with six human recombinant cytochromes P450 (1A2, 2A6, 3A4, 2C9, 2D6, and 2E1). (A) Patient 12 (see Figure 3): autoimmune hepatitis, LKM-1 positive, autoantigen CYP2D6. (B) Patient 7 (see Table 1 and Figures 1 and 3): APS-1, LKM positive, autoantigen CYP2A6. (C) Patient 10 (see Table 1 and Figures 1-3): APS-1, LKM positive, autoantigen CYP2A6. (D) Patient 11 (see Table 1 and Figures 1 and 3): APS-1, LKM positive, autoantigen CYP2A6. Markers on the left indicate the molecular weights in kilodaltons.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions