1. Corona cell RNA sequencing from individual oocytes revealed transcripts and pathways linked to euploid oocyte competence and live birth 
Jason C. Parks, Alyssa L. Patton, Blair R. McCallie, Darren K. Griffin, William B. Schoolcraft, Mandy G. Katz-Jaffe 
Reproductive BioMedicine Online 
Volume 32, Issue 5, Pages (May 2016)
DOI: /j.rbmo
Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Volcano plot of transcripts from corona cells associated with live birth compared with negative implantation. Each dot represents a gene; red dots are significantly differentially expressed genes at P < 0.05 and fold change ≥2. Up-regulated genes are shown as positive values on the x-axis, and down-regulated genes are shown as negative values on the x-axis.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

3. Figure 2
Fold change in negative implantation relative to live birth. Quantitative real-time polymerase chain reaction validation on additional individual corona cell samples displayed significantly increased differential expression of DUSP16 and TNSFRSF10A in negative implantation samples relative to live birth samples (P < 0.05), and a trend towards increased differential expression of TXNIP. P-values were calculated using REST-2009© software, hence no error bars are present.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

4. Figure 3
Fold change in negative implantation relative to live birth. WNT-canonical pathway genes AXIN1, GSK3B and APC were validated by quantitative real-time polymerase chain reaction on additional individual corona cell samples and showed significantly increased expression associated with negative implantation relative to live birth samples (P < 0.05). P-values were calculated using REST-2009© software, hence no error bars are present.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

5. Figure 4
The Wnt-signalling pathway. Wnts are secreted extracellular signalling molecules that exert local control over diverse developmental processes including cell-fate specification, differentiation and regulation of cell-cell interactions. (A) As Wnt binds to cell surface receptors, it turns off beta-catenin destruction, which in turn stabilizes beta-catenin, allowing translocation into the nucleus and activation of Wnt target gene transcription. Wnt signalling was identified as an enriched pathway with increased differentially expressed transcripts in association with live birth (P < 0.05); (B) In the absence of Wnt signalling, increased expression of AXIN1, APC and GSK3 leads to the degradation of beta-catenin. The beta-catenin destruction complex was observed in association with negative implantation, and decreased differentially expressed transcripts (P < 0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions