1. Fig. 1. Transfer of miR-223 during neutrophil-epithelial cell interactions.
Transfer of miR-223 during neutrophil-epithelial cell interactions. (A) Coculture setup for human neutrophils (PMNs) and human pulmonary epithelial cells. (B) Expression of miRNA in human epithelial cells after coculture of HPAEpiC with activated human PMNs (means ± SEM; n = 4). (C) hsa–miR-223 expression after coculture of HPAEpiC with activated human PMNs (means ± SEM; n = 6). (D and E) Expression of miR-223 in HPAEpiC after exposure of HPAEpiC cells to (D) fMLP or (E) LPS [means ± SEM; n = 2 for control (Ctr) group or n = 3 for fMLP and LPS groups]. (F) Epithelial cell miR-223 target vector luciferase activity after coculture of activated human PMNs with transfected pulmonary epithelial cells (Calu-3); data are normalized to control vector activity and compared to no coculture (means ± SEM; n = 3 independent experiments). (G) Setup for murine coculture. (H) mmu–miR-223 expression in mouse pulmonary epithelial (MLE-12) cells after coculture with activated murine PMNs derived from wild-type (WT) or miR-223−/y mice (means ± SEM; n = 11 for the control group, n = 6 for mouse WT PMNs, and n = 7 for miR-223−/y PMNs). (I) mmu–miR-223 expression in MLE-12 cells or E-10 cells after coculture with activated murine PMNs (means ± SEM; n = 7 for MLE-12 control group or n = 8 for all other conditions) [*P < 0.05, Student’s t test or analysis of variance (ANOVA)]. n.s., not significant.
Viola Neudecker et al., Sci Transl Med 2017;9:eaah5360
Published by AAAS