1. The Intracellular Highway
Protein synthesis and Transport
GLOWING MARMOSETS CLASS 2
2/13/10
S. BACHAR AND R. ZACHARIAH

2. Each define an organelle  tie it all together at the end.
Organelles

3. Transcription and Translation
What does the m in mRNA stand for? Why?
Transcription and Translation

4. Protein Targeting
Protein Targeting

5. Activity!
You are a scientist and you notice that most proteins that end up in the rough endoplasmic reticulum have the same sequence on one end of the protein.
Design an experiment to prove your hypothesis – either the signal sequence is enough to get any protein into the ER or not.

6. Membrane Proteins How do they get there?

7. Finding Order: Temperature Sensitive Mutants

8. Finding Order: Temperature Sensitive Mutants

9. Jelly Fish!
AEQUOREA VICTORIA

10. µm µm
1 - 2 cm
WHAT IS MICROSCOPY?

11. Bright Field and stains

12. Fluorescence

13. Using Antibodies

14. GFP-Tagging

15. Organelle Staining

16. SEM/TEM