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Heterogeneity of BRAF, NRAS, and TERT Promoter Mutational Status in Multiple Melanomas and Association with MC1R Genotype  Cristina Pellegrini, Lucia.

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Presentation on theme: "Heterogeneity of BRAF, NRAS, and TERT Promoter Mutational Status in Multiple Melanomas and Association with MC1R Genotype  Cristina Pellegrini, Lucia."— Presentation transcript:

1 Heterogeneity of BRAF, NRAS, and TERT Promoter Mutational Status in Multiple Melanomas and Association with MC1R Genotype  Cristina Pellegrini, Lucia Di Nardo, Gianluca Cipolloni, Claudia Martorelli, Marina De Padova, Ambra Antonini, Maria Giovanna Maturo, Laura Del Regno, Sara Strafella, Tamara Micantonio, Pietro Leocata, Ketty Peris, Maria Concetta Fargnoli  The Journal of Molecular Diagnostics  Volume 20, Issue 1, Pages (January 2018) DOI: /j.jmoldx Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Representative cases of BRAFV600E (VE1) immunostaining. A: Weak staining intensity with heterogeneous distribution. B: Moderate staining intensity with heterogeneous distribution. C: Strong staining intensity with homogeneous distribution. Original magnification, ×20. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 BRAFV600E (VE1) staining in melanoma and associated nevus. A and B: Lesion with concordant positive BRAFV600E in melanoma and nevus: hematoxylin and eosin (H&E) staining (A) and BRAFV600E (VE1) staining (B). C and D: Lesion with concordant BRAF wild type in melanoma and associated nevus: H&E staining (C) and negative BRAFV600E (VE1) staining (D). Original magnification: ×10 (A and B); ×20 (C and D). The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Illustrative cases of melanomas that show concordant or discordant findings between molecular analysis and immunohistochemistry. Left column: Melanoma showing BRAF wild type by molecular testing but weak and heterogeneous staining by BRAFV600EVE1 antibody. Middle column: Melanoma with the BRAFV600E mutation by molecular testing and moderately and heterogeneously immunostained by immunohistochemistry (IHC). Right column: Melanoma BRAFV600E mutated according to molecular testing with strong and homogeneous immunostaining by IHC. Original magnification, ×10. A, adenine; c., chromosome nucleotide; T, thymine; WT, wild-type. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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