1. *** *** Supplemental figure 1 1.2 1.0 0.8 NR4As/ cyclophilin mRNA 0.6
0.4
0.2
***
***
NOR1
Nurr1
Nur77
Supplemental figure 1

2. non specific complexesA B
Hypertonic buffer
Hypertonic buffer
Anti-STAT6 Ab RM M2 RM M2 1 2 3 4 5 6 7 8 9 10 11
super-shift
STAT6
non specific complexes
NOR1 STAT6-RE
NOR1
STAT6-RE mut
consensus
STAT6-RE
NOR1
STAT6-RE
Supplemental figure 2

3. A B ns ns Supplemental figure 3 scrambled siNOR1
1.6
4.0
siNOR1
1.4
3.5 ns ns
1.2
3.0
1.0
2.5
Nur77 / cyclophilin mRNA
Nurr1 / cyclophilin mRNA
0.8
2.0
0.6
1.5
0.4
1.0
0.2
0.5
Supplemental figure 3

4. A B C D ** ** ** ** * ** ** ** ** E F G * * * * ** * ** **
1.2
1.0
3.5
3.0
1.0
0.8
Scrambled
Smart pool
SiNOR1 -1
SiNOR1-2 ** **
2.5
0.8 ** ** * ** **
0.6
2.0
NOR1
PPARg / cyclophilin mRNA
F13A1 / cyclophilin mRNA
0.6
MR / cyclophilin mRNA ** **
0.4
1.5
b-actin
0.4
1.0
0.2
0.2
0.5
scrambled
smart pool
siNOR1-1
siNOR1-2
scrambled
smart pool
siNOR1-1
siNOR1-2
scrambled
smart pool
siNOR1-1
siNOR1-2 E F G 10
1.6
1.2 9
1.4
p=0.11 8
1.0
1.2 * 7 *
0.8 * * **
1.0 6 * **
CD200R / cyclophilin mRNA 5
IL-10 / cyclophilin mRNA **
IL-1Ra / cyclophilin mRNA
0.8
0.6 4
0.6 3
0.4
0.4 2
0.2
0.2 1
scrambled
smart pool
siNOR1-1
siNOR1-2
scrambled
smart pool
siNOR1-1
siNOR1-2
scrambled
smart pool
siNOR1-1
siNOR1-2
Supplemental figure 4

5. A B ns ns Supplemental figure 5 scrambled siNOR16
1.8 ns ns
1.6 5
1.4 4
1.2
CD163 / cyclophilin mRNA
AMAC1 / cyclophilin mRNA
1.0 3
0.8 2
0.6
0.4 1
0.2
Supplemental figure 5

6. A B C RM M2 F13A1 RM M2 IL-1Ra RM M2 IL-10 EGR2B EGR2B PU.1 PU.1 CEBP
ttacAAGGtcagtaa
EGR2B
PU.1
CEBP
H3K9ac RM M2
F13A1 RM M2
actcAAGGtcacact
gtccAAGGtcacaca
gttcAAGGtcacacg
cataAAGGtcagagt
taaaGAGGtcaatag
cttcAAGGtcatcca
gagaAAGGtcctgag
EGR2B
PU.1
CEBP
H3K9ac
IL-1Ra A B
EGR2B
PU.1
CEBP
H3K9ac RM M2
gagaAAGGacaggaa
cagaAAGGtgaagaa
agagAAGGtcactca
tccaTAGGtcacaca
tcatAAGGtcaggag
cccaTAGGtcatgtc
IL-10 C
Supplemental figure 6

7. A B Supplemental figure 7 donor 1 donor 2 donor 3 donor 4 donor 5
564 up-regulated genes
MMP9
down-regulated genes
up-regulated genes
NOR1
617 down-regulated genes
Supplemental figure 7

8. A
P values
<0.05
>0.05 B
Supplemental Figure 8

9. ** * A B D C Supplemental Figure 9 CH25H MAP2K6 FCGR2B F13A1 scrambled
0.2
0.4
0.6
0.8
1.0
1.2
gene/cyclophilin mRNA ** * A B D C
CH25H
MAP2K6
FCGR2B
F13A1
scrambled
siNOR1
Supplemental Figure 9

10. *** *** *** Supplemental Figure 10 scrambled smart pool siNOR1-16 6
*** 5 5
*** 4 4
***
MMP9 / cyclophilin mRNA 3 3 2 2 1 1
scrambled
smart pool
siNOR1-1
siNOR1-2
Supplemental Figure 10

11. MMP9 RM M2 EGR2B PU.1 CEBP H3K9ac Supplemental Figure 11
gcaaAAGGccaagga
EGR2B
PU.1
CEBP
H3K9ac RM M2
MMP9
Supplemental Figure 11

12. A B ns ns Supplemental figure 12 scrambled siNOR18 40
scrambled ns
siNOR1 ns 6 30
MMP7 / cyclophilin mRNA
MMP8 / cyclophilin mRNA 4 20 2 10
Supplemental figure 12

13. Supplemental table 1. Primer sequences

14. Supplemental table 2. Genes up-regulated in siNOR1 M2 macrophages compared to scrambled siRNA transfected cells
Gene symbol
Gene title
Fold change
p-value
TPM4
CCNA1
STX12
ZNF616
ZNF117
CD44
MMP9
HDAC9
PLS3
ZDHHC16
tropomyosin
cyclin A1
syntaxin12
zinc finger protein 616
zinc finger protein 117
CD44 molecule
matrix metallopeptidase 9
histone deacetylase 9
plastin 3
zinc finger, DHHC-type 16
1.7
1.67
1.6
1.56
1.55
1.54
1.53
1.51
1.5
1.9*10-2
3.7*10-2
5*10-3
8*10-3
3*10-3
9*10-3
4.4*10-2
1.5*10-2

15. Supplemental table 3. Genes down-regulated in siNOR1 M2 macrophages compared to
scrambled siRNA transfected cells
Gene symbol
Gene title
Fold change
p-value
NR4A3
CXCL10
NEXN
FLT1
TNFS10
WWTR1
IGF2BP3
CH25H
SLC51B
SMAD5
nuclear receptor 4 A 3 (NOR1)
CXC motif chemokine 10
nexilin (F actin-binding protein)
fms-related tyrosine kinase 1
TNF ligand superfamily, member 10
ww-domain-containing transcription regulator protein 1
insulin-like growth factor 2 mRNA binding protein 3
cholesterol-25-hydrolase
solute carrier family 51, b subunit
smad family member 5
-2.44
-2.2
-2.1
-1.92
-1.82
-1.76
-1.7
-1.69
-1.67
2*10-4
2.5*10-2
4.8*10-2
2.8*10-4
3*10-2
2*10-3
8*10-3
5*10-3
1.3*10-2
3*10-3