1. Peixoto et al, Supplementary Figure 4.1 A(top)
ROP17-HA
ROP19-HA
ROP20-HA
ROP23-HA
Figure S ROPK Localization. A, Most ROPK proteins localize to the rhoptries and parasitophorous vacuole, as detected by co-localization of antibodies to HA-tagged antigen (green) and native ROP2/3/4 (red); blue = DAPI (host nuclei are visible in some images). B, ROP21 and ROP22 do not co-localize with rhoptry proteins, but are secreted into the parasitophorous vacuole and are observed within the host cytoplasm late during infection (Fig. 3).
ROP25-HA
ROP39-HA
ROP40-HA
anti-ROP2
merge
anti-HA
phase

2. Peixoto et al, Supplementary Figure 4.1 B(top)
ROP21-HA
ROP22-HA
Figure S ROPK Localization. A, Most ROPK proteins localize to the rhoptries and parasitophorous vacuole, as detected by co-localization of antibodies to HA-tagged antigen (green) and native ROP2/3/4 (red); blue = DAPI (host nuclei are visible in some images). B, ROP21 and ROP22 do not co-localize with rhoptry proteins, but are secreted into the parasitophorous vacuole and were also observed within the host cytoplasm late during infection (Fig. 3).
phase
anti-HA
anti-ROP2
merge