Presentation is loading. Please wait.

Presentation is loading. Please wait.

Gorav Ailawadi, MD, Christopher W. Moehle, BS, Hong Pei, MD, Sandra P

Published byΕκάτη Κουρμούλης Modified over 6 years ago

Similar presentations

Presentation on theme: "Gorav Ailawadi, MD, Christopher W. Moehle, BS, Hong Pei, MD, Sandra P"— Presentation transcript:

1 Smooth muscle phenotypic modulation is an early event in aortic aneurysms 
Gorav Ailawadi, MD, Christopher W. Moehle, BS, Hong Pei, MD, Sandra P. Walton, BS, Zequan Yang, MD, PhD, Irving L. Kron, MD, Christine L. Lau, MD, Gary K. Owens, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 138, Issue 6, Pages (December 2009) DOI: /j.jtcvs Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

2 Figure 1 A, Elastase perfusion of the infrarenal abdominal aorta. B, An explanted abdominal aortic aneurysm 14 days after elastase perfusion. C, Graph demonstrating aortic aneurysm formation at 14 days after elastase (and not saline) perfusion. Importantly, at 7 days, no differences in aortic diameter are evident between elastase- and saline-treated animals. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

3 Figure 2 Quantitative polymerase chain reaction analysis of smooth muscle marker genes at 7 and 14 days after elastase/saline perfusion. SM22α and SM α-actin expression is downregulated in elastase-treated aortas at 7 and 14 days after perfusion. Standard error bars are shown. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

4 Figure 3 Histochemistry and immunohistochemistry in mice 7 days after elastase or saline perfusion. Little differences in elastin are evident by means of Movat staining, with intact elastin in both treatment groups. Some early degradation might be present in regions of the aorta (blue arrowheads) after elastase perfusion compared with that seen after saline perfusion. Expression of matrix metalloproteinase (MMP) 2 and 9 appears to be upregulated at 7 days after elastase compared with that seen after saline. Minimal activated (cleaved) caspase-3 staining is present in either group (brown positive stain, methyl green nuclear counterstain). Images were obtained at 25× magnification, except for caspase-3, which was obtained at 10× magnification. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

5 Figure 4 Histochemistry and immunohistochemistry in mice 14 days after elastase or saline perfusion. Movat staining demonstrated destruction of the medial elastin layer in elastase-treated animals. SM α-actin and SM22α (arrowheads) are downregulated compared with that seen in saline controls. Expression of matrix metalloproteinase (MMP) 2 and 9 is markedly increased in elastase-treated animals. There is little activated (cleaved) caspase-3 staining in either elastase- or saline-treated animals (brown staining positive, methyl green nuclear counterstain). Images were obtained at 25× magnification, except for caspase-3, which was obtained at 10× magnification. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

6 Figure 5 Quantitative polymerase chain reaction in human ascending aortic aneurysms and control ascending aortic tissue. SM α-actin expression is significantly decreased in ascending aortic aneurysms, whereas matrix metalloproteinase (MMP) 2 expression is increased compared with that seen in control aortas. Standard error bars are shown. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

7 Figure 6 Immunohistochemistry in human ascending and abdominal aortic aneurysms (AA) and control ascending aortas. SM α-actin and SM22α are downregulated in aortic aneurysms compared with that seen in control aortas. Expression of matrix metalloproteinase (MMP) 2 and 9 is increased in aneurysmal tissue. Apoptosis (caspase-3) is evident in ascending and abdominal aortic aneurysms and, to a lesser extent, in control aortas. All images were obtained at 10× magnification. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Download ppt "Gorav Ailawadi, MD, Christopher W. Moehle, BS, Hong Pei, MD, Sandra P"

Similar presentations

Of mice and men and surgical transcatheter aortic valve insertion

Of mice and men and surgical transcatheter aortic valve insertion
Manuel J. Antunes, MD, PhD, DSc 

Manuel J. Antunes, MD, PhD, DSc 
Azelnidipine suppresses the progression of aortic aneurysm in wild mice model through anti-inflammatory effects  Hirotsugu Kurobe, MD, PhD, Yuki Matsuoka,

Azelnidipine suppresses the progression of aortic aneurysm in wild mice model through anti-inflammatory effects  Hirotsugu Kurobe, MD, PhD, Yuki Matsuoka,
Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,
Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 
Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 
Pharmacologic blockade and genetic deletion of androgen receptor attenuates aortic aneurysm formation  John P. Davis, MD, Morgan Salmon, PhD, Nicolas.

Pharmacologic blockade and genetic deletion of androgen receptor attenuates aortic aneurysm formation  John P. Davis, MD, Morgan Salmon, PhD, Nicolas.
Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.

Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.
Response of plasma matrix metalloproteinases and tissue inhibitor of metalloproteinases to stent-graft surgery for descending thoracic aortic aneurysms 

Response of plasma matrix metalloproteinases and tissue inhibitor of metalloproteinases to stent-graft surgery for descending thoracic aortic aneurysms 
Smooth muscle cells from abdominal aortic aneurysms are unique and can independently and synergistically degrade insoluble elastin  Nathan Airhart, MD,

Smooth muscle cells from abdominal aortic aneurysms are unique and can independently and synergistically degrade insoluble elastin  Nathan Airhart, MD,
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Loss of STAT1 is associated with increased aortic rupture in an experimental model of aortic dissection and aneurysm formation  Matthew J. Eagleton, MD,

Loss of STAT1 is associated with increased aortic rupture in an experimental model of aortic dissection and aneurysm formation  Matthew J. Eagleton, MD,
Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,

Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,
Vascular smooth muscle cell peroxisome proliferator-activated receptor-γ deletion promotes abdominal aortic aneurysms  Milton Hamblin, PhD, Lin Chang,

Vascular smooth muscle cell peroxisome proliferator-activated receptor-γ deletion promotes abdominal aortic aneurysms  Milton Hamblin, PhD, Lin Chang,
Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection  Lixin Wang, MD, PhD, Jing Zhang, MD, PhD,

Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection  Lixin Wang, MD, PhD, Jing Zhang, MD, PhD,
Bone marrow–derived MCP1 required for experimental aortic aneurysm formation and smooth muscle phenotypic modulation  Christopher W. Moehle, MS, Castigliano.

Bone marrow–derived MCP1 required for experimental aortic aneurysm formation and smooth muscle phenotypic modulation  Christopher W. Moehle, MS, Castigliano.
The fibrillin-1 hypomorphic mgR/mgR murine model of Marfan syndrome shows severe elastolysis in all segments of the aorta  Simon Schwill, Philipp Seppelt,

The fibrillin-1 hypomorphic mgR/mgR murine model of Marfan syndrome shows severe elastolysis in all segments of the aorta  Simon Schwill, Philipp Seppelt,
Indomethacin inhibits expansion of experimental aortic aneurysms via inhibition of the cox2 isoform of cyclooxygenase  Manuel Miralles, MD, William Wester,

Indomethacin inhibits expansion of experimental aortic aneurysms via inhibition of the cox2 isoform of cyclooxygenase  Manuel Miralles, MD, William Wester,
Differential expression of elastin assembly genes in patients with Stanford Type A aortic dissection using microarray analysis  Bernice L.Y. Cheuk, PhD,

Differential expression of elastin assembly genes in patients with Stanford Type A aortic dissection using microarray analysis  Bernice L.Y. Cheuk, PhD,
Doxycycline inhibition of aneurysmal degeneration in an elastase-induced rat model of abdominal aortic aneurysm: Preservation of aortic elastin associated.

Doxycycline inhibition of aneurysmal degeneration in an elastase-induced rat model of abdominal aortic aneurysm: Preservation of aortic elastin associated.

Similar presentations

Ads by Google