1. Terse ImageJ Manual
When you startup ImageJ, the main console will appear on your screen. The
relevant features of the main console for this activity are summarized below.
File – managers opening, saving, closing of image files
Edit – undo button, cut, copy, and paste
Analyze – measure toolbar, set measurements
Image – adjust picture setting, properties, and crop pictures

2. Loading an Image 1. Click File > Open (Crtl+O)
2. Browse for image, select image, click Open
3. Selected image will appear in new window

3. Calibrate an Image 1. Click Image > Properties
2. Enter image calibration factor for pixel width, heigth, & depth. Change unit of length to microns
3. Check input and click OK

4. Image Measurements
1. Length Measurements: Right click line icon for length measurement options
2. Click and drag over feature of interest. Click Analyze > Measure (Crtl +M)
3. Measurement will appear in new Results window
Feature

5. Image Measurements Cont.
4. Area Measurements: Choose from following options on Main Console
Freehand
Polygonal Area
Elliptical Area
Rectangular Area
5. Outline area of interest. Right click to close outline.
6. Repeat Steps 2- 3 to record area measurement.

6. This completes the Terse ImageJ Manual
This completes the Terse ImageJ Manual. If more program features are needed, but consult official ImageJ manual online.
Link to ImageJ Documentation