1. (A) Schematic representation of kalata B1 showing the cyclic cystine knot, the amino acid sequence in single letter code, and the regions used for oligonucleotide primer design (shaded).
(A) Schematic representation of kalata B1 showing the cyclic cystine knot, the amino acid sequence in single letter code, and the regions used for oligonucleotide primer design (shaded). (B) The primers used in the PCR reactions. I represents inosine, Y represents C or T, and R represents A, C, T, or G. The introduced restriction enzyme sites are in italics. (C) Amino acid sequence of the protein encoded by the Oak1 clone. The sequence corresponding to the PCR product obtained with the Kal2 and oligo-dT primers is shaded.
Cameron Jennings et al. PNAS 2001;98:19:
©2001 by National Academy of Sciences