1. AP Bio: Tuesday, 9.27.11 Metabolic Reactions & Enzymes
Homework:
Pass PS 4 to the front to be collected
PS 5 due a week from today
Do Now:
Today is a lecture/notes day… take out a notebook (title for today is at the top of the slide)
The following terms can be divided into two logical categories. Split them as best you can into two lists.
Endergonic Synthesis Condensation
Exergonic Decomposition Hydrolysis
Catabolism Cellular respiration
Anabolism Photosynthesis

2. Today’s Agenda & Goals Agenda: Goals:
Lecture on Metabolic Reactions & Enzymes
Goals:
Distinguish between endergonic and exergonic reactions
Describe the role of ATP in metabolic reactions
Explain the role of enzymes as biological catalysts

3. Video #1: Energy in Living Things
1st Law of Thermodynamics: Energy can’t be created or destroyed, it only changes forms.
Forms of energy –
Potential (stored) – includes chemical energy (energy held in chemical bonds) (ex: glucose, ATP, fatty acids)
Kinetic (movement) – includes heat energy (the most random/disordered form)
2nd Law of Thermodynamics: Every energy transfer involves the “loss” of some energy as heat (so the total amount of “disordered” energy increases overall)

4. Two categories of metabolic reactions…
Hydrolysis
Decomposition
Catabolism
Cellular respiration
Exergonic
Condensation
Synthesis
Anabolism
Photosynthesis
Endergonic

7. How do cells power endergonic reactions?
ATP

9. ATP – Your cells’ rechargeable battery

11. The ATP/ADP Cycle is coupled with other cellular reactions

13. AP Bio: Wednesday, 9.28.11 Metabolic Reactions & Enzymes (Day 2)
Homework:
Lab 2A Analysis Questions (page 3)
Lab 2B and 2C Pre-Lab (page 4)
Do Now:
On the Lab 2 packet, read the Overview and answer the Pre-Lab Questions on pages 1-2.
Today’s Agenda:
Lab 2 Pre-Lab and Demonstration
Lecture (continued) – Enzymes

14. Video #2: Controlling Cellular Metabolism

15. Even exergonic reactions require activation energy.

16. Catalysts lower the energy barrier.

17. How do enzymes work?
Specificity – every enzyme’s shape matches its substrate
Substrate binds to enzyme’s active site
Induced fit – enzyme and substrate both change shape slightly
Bond strain lowers the EA needed to break substrate bonds
Substrate turns to product; enzyme remains unchanged

18. Enzyme shape is essential!

19. AP Bio: Thursday, 9.29.11 Lab 2: Enzyme Catalysis
Homework:
Lab 2 Graphs and Analysis Questions
Do Now:
Take out Lab 2 and turn to page 3.
Today’s Goals:
Quantitatively measure the rate of an enzyme-catalyzed reaction
Experimentally determine how temperature, pH, and substrate concentration affect the rate of an enzyme-catalyze reaction

20. AP Bio: Thursday, 9.29.11 Lab 2: Enzyme Catalysis
HW: Lab 2 (graphs and analysis questions) due Tuesday
Prob Set 5 now due Wed. not Tuesday!
Agenda:
Discussion: Part A and Pre-Lab for Parts B and C (5 min)
Demonstration of lab set-up (5 min)
Lab! Part B, then Part C
Today’s Goals:
Quantitatively measure the rate of an enzyme-catalyzed reaction
Experimentally determine how temperature, pH, and substrate concentration affect the rate of an enzyme-catalyze reaction

21. AP Bio: Tuesday, 10/4/11 Lab Debrief; Enzyme Regulation
Homework:
Problem Set 5 due Wednesday
Unit Test on Ch. 1-5 and 8 on TUESDAY (see review sheet)
Do Now:
Take out Lab 2 and your graphs
Today’s Goals:
Analyze data from Lab 2
Explain how enzyme activity is regulated in cells

22. Video of Peroxidase in Action…
See Starr cd ch. 5 catalase

23. Videos on Protein Shape…

24. Enzyme Regulation

25. Competitive Inhibition

26. Non-Competitive Inhibition

27. Allosteric Regulation

28. Allosteric Regulation
A regulator must bind in order to activate the enzyme
Similar to, but opposite from, non-competitive inhibition

29. FEEDBACK INHIBITION

30. AP Bio: Wednesday, 10.5.11 Unit 1 Test Review!
Homework:
Study for tomorrow’s test!
Do Now:
Take out PS 5
Take minutes to answer the practice questions in the packet
Objectives:
Review the material on the Unit 1 Test
Agenda:
Practice questions / Hand back PS 4
Discuss practice questions & other review topics
Hand in PS 5