1. Reticulocyte Detection
Sysmex XE-2100
Reticulocyte Detection

2. Reticulocyte Methodology
semiconductor laser flow cytometry method.
RET-SEARCH (II) (diluent)
RET-SEARCH (II) dye (stain)
passed through the laser flow cytometer for analysis.
Counted using forward light scatter and side fluorescence.

3. Flow Cytometry Forward light scatter - size.
Side fluorescence - nuclear content.

4. RETIC Channel Explained

5. Therefore the RETIC Scattergram …

6. RETIC “Differential” Retic RNA content decreases on maturation.
Thus fluorescence decreases with maturation.
The XE2100 resolves the differential stages by fluorescence intensity.