1. Methods Objectives Results Conclusions References
Nano Curcumin as a promising agent for diminution of oxymetholone induced reprotoxicity in female mice
Seyedeh Fatemeh Vaezzadeh1, Samad Zare1, Gholamreza Najafi 2, Ali Shalizar Jalali 2
1 Department of Biologic Sciences, Faculty of Science, Urmia University, Urmia, Iran
Objectives
Results
Methods
Results showed significantly reduction in IVF outcomes in OXM received groups versus other groups(p<0.05). MII oocyte number was decreased in OXM group and its improvement was not observed in treated groups. Cleavage rate on day 2 was significantly improved in Vit-E treated group only (p<0.05) and cleavage rate at blastocyst stage was not significantly improved in the treated groups.
The aim of the present study was to Vitamin E and Nano- curcumin in sole and simultaneous forms of administration partially provoke oxymetholone -reduced fertilizing potential.
In order to perform current study, thirty adult female mice were randomly categorized into experimental and control groups. The experimental group subdivided into five groups, which orally received vitamin E-sole (100 mg/kg.bw), NCMN-sole (15 mg/kg.bw), Oxymetholone-sole (5 mg/kg.bw), vitamin E (100 mg/kg.bw) +oxymetholone (5 mg/kg.bw), NCMN (15 mg/kg.bw) + Oxymetholone (5 mg/kg.bw). After 21 days, the oocytes were picked-up by inducing super ovulation (10 IU PMSG and 10 IU hCG) and underwent in vitro fertilization process using fresh sperms from intact mice. Then, Twenty-four hours after insemination, oocytes were monitored by an inverted microscope and formation of the pronuclei and polar bodies was recorded to evaluate fertilization rate. Blastocyst development rate was evaluated by determining the number of embryos that had reached the blastocyst stage after 72 hours’ incubation.
Conclusions
The present study reveals that the OXM impacts negatively on IVF outcomes of mice oocytes and demonstrated that Vitamin E and Nanocurcumin could not overcome these effects. Although our treatments showed no improvement in IVF outcomes of OXM-induced mice, but Vit-E had better results which can use in strategies against destructive effects of OXM.
References