1. iGEM 2005 & 2006 http://arep.med.harvard.edu/SBP/iGEM/iGEM_2004.ppt

2. 2007 Pre-iGEM meetings * Overview * Parts & Devices
* Case study (& Voigt review)
* New Strengths
2: list of working parts & systems
3. Brainstorm projects
4. Show how to design & order parts.

3. Biobricks
Proposal that the 2007 iGEM awards be biological, useful, & CO2-neutral.

4. The 2004 iGEM poster was synthetic, but not biological
( an artifact of light sensitive dyes)
Privately rehabilitated in time for 2005 iGEM jamboree & then publication:
Levskaya A, et al. Nature 2005 Nov 24 Synthetic biology:
engineering
E.coli to see light.
Low contrast:
Input: Red light / phycocyanobilin
Out: LacZ / S-gal
(3,4-cyclohexenoesculetin-
-D-galactopyranoside):

5. Voigt 2006 Current Opinion in Biotechnology 2006, 17:548–557
Light Input/Output
Voigt Current Opinion in Biotechnology 2006, 17:548–557

6. Possible alternatives for light I/O
‘Caged’ substrates for light input
Multiple wavelengths
Luciferase output

7. Potential strengths barely tapped
Combinatorial constructions
Selections
Surface expression
Homologous recombination

8. .