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Rhinoconjunctival sensitization to hydrolyzed wheat protein in facial soap can induce wheat-dependent exercise-induced anaphylaxis  Yuma Fukutomi, MD,

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Presentation on theme: "Rhinoconjunctival sensitization to hydrolyzed wheat protein in facial soap can induce wheat-dependent exercise-induced anaphylaxis  Yuma Fukutomi, MD,"— Presentation transcript:

1 Rhinoconjunctival sensitization to hydrolyzed wheat protein in facial soap can induce wheat-dependent exercise-induced anaphylaxis  Yuma Fukutomi, MD, Yasuharu Itagaki, PhD, Masami Taniguchi, MD, PhD, Akemi Saito, BSc, Hiroshi Yasueda, PhD, Takuya Nakazawa, MD, PhD, Maki Hasegawa, MD, PhD, Hiroyuki Nakamura, MD, PhD, Kazuo Akiyama, MD  Journal of Allergy and Clinical Immunology  Volume 127, Issue 2, Pages e3 (February 2011) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Comparison of IgE reactivity to HWP and wheat protein fractions (A) and CAP-IgE to recombinant ω5-gliadin (B) between patients with WDEIA accompanied by rhinoconjunctival contact allergy to HWP (closed circles) and patients with conventional WDEIA (open circles). Alb, Albumin-globulins; Gli, gliadins; Glu, glutenins. The number of patients with negative test results is indicated below the dotted cutoff line (0.35 U/mL). Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 SDS -PAGE (A) and IgE immunoblotting (B) of HWP
SDS -PAGE (A) and IgE immunoblotting (B) of HWP. M, Marker weight; P, HWP (40 μg dry weight/lane), electrophoresed on 14% gel stained with Coomassie Brilliant Blue; lanes H1-H5, IgE reactivity of sera of patients numbered H1 to H5 in Table E1, detected by using the enhanced chemiluminescence system after goat antihuman IgE–horseradish peroxidase conjugate incubation; NC, negative control. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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