1. Gene transfer of heat shock protein 70 protects lung grafts from ischemia-reperfusion injury 
Masafumi Hiratsuka, MD, Bassem N Mora, MD, Motoki Yano, MD, Thalachallour Mohanakumar, PhD, G.Alexander Patterson, MD 
The Annals of Thoracic Surgery 
Volume 67, Issue 5, Pages (May 1999)
DOI: /S (99)

2. Fig 1
(A) Western blot analysis of lungs transfected with various titers of adenovirus-HSP70 5 days after injection (lanes 1, 2: saline; lanes 3, 4: 5 × 109 pfu; lanes 5, 6: 1 × 1010 pfu; lanes 7, 8: 2 × 1010 pfu). The overexpression of HSP 70 was detected even at low titer (5 × 109 pfu). Each bar represents mean ± SE of spectrophotometric analysis of blots, two samples within each group. (B) Western blot analysis of lungs transfected with 5 × 109 pfu of adenovirus-HSP70 and sacrificed various periods after injection (lane 1: positive control; lanes 2, 3: sacrifice 0 hours; lanes 4, 5: 6 hours; lanes 6, 7: 24 hours; lanes 8, 9: 72 hours). Stronger expression of HSP70 was detected at 24 hours after intravenous injection. Each bar represents mean ± SE of spectrophotometric analysis of blots, two samples within each group.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

3. Fig 2
Western blot analysis of HSP70 in lung tissue at the time of harvest. Lane 1 represents a positive control, lanes 2 through 4 represent group 3, lanes 5 through 7 represent group 2, and lanes 8 through 10 represent group 1. Each bar represents mean ± SE of spectrophotometric analysis of blots, three samples within each group.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

4. Fig 3
Western blot analysis of HSP90 at the time of harvest. Lane 1 represents a positive control, lanes 2 through 4 represent group 3, lanes 5 through 7 represent group 2, and lanes 8 through 10 represent group 1. Each bar represents mean ± SE of spectrophotometric analysis of blots, three samples within each group.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

5. Fig 4
The mean arterial oxygenation (Pao2) 24 hours after reperfusion. Pao2 in group 1 was superior in comparison with group 3 (p = ) and group 2 (p = ).
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

6. Fig 5
Wet to dry weight ratio was calculated at 24 hours after reperfusion. The ratio of left transplanted lungs in group 1 was significantly less than the other groups.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

7. Fig 6
Myeloperoxidase (MPO) activity was significantly less in transplanted lungs in group 1 compared with the other groups.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )