1. The Light Microscope

2. Learning goals We are learning the parts of a microscope.
We are learning to accurately view objects through a microscope.

3. Microscopes In high school we use light microscopes.
They use light to illuminate images.
There are other microscopes called electron microscopes which use a beam of electrons.
These give amazing views but each cost hundreds of thousands of dollars.

4. Red Blood Cell Images
Light microscope
Electron microscope

5. Using a Microscope What is the field of view?
The white circle that we see when we look under a microscope.

6. Special Parts of a Microscope
Ocular Lens: Or the eyepiece lens, it is what the scientist looks through to observe the prepared slide on the stage.
Also magnifies the image 10 times the naked eye (10x).
Objective Lens: Lens that provides additional magnification. Three types:
Low (4x), Medium (10x), High (40x)
All three lenses on a revolving nosepiece.

7. Diaphragm: Controls the amount of light entering the slide.
Adjustment Knobs: Provides focus control. Two types:
Coarse: Large increases in focus
Fine: Smaller increases in focus to clarify image.

8. The Compound Microscope
Ocular Lens
High powered objective lens
Low powered objective lens
Medium powered objective lens
Arm
Clips
Diaphragm
Stage
Coarse Adjustment Knob
Light source
Fine Adjustment Knob
Base

9. Calculating Magnification
Eg.1: What is the magnification of a sample seen clearly under the medium powered objective lens (10x)?
Ocular Lens =
Med Obj Lens =
Total Magnification =
The object is magnified 100x that of the naked eye.
10x
10x x
*Take the product of both lenses
100x

10. Drawing Scientific Drawings
Guidelines to following when doing microscope drawings:
Circle indicating field of view
Title at top of drawing
Magnification at bottom of drawing
Draw using pencil ONLY
Label significant points using a ruler

11. Learning goals We are learning the parts of a microscope.
We are learning to accurately view objects through a microscope.