1. Chlorophyll a Measurement

2. First, calculate amount of Chl a Ca = 11.85A665 - 1.54A647 - 0.08A630
Next, calculate mg Chl a / m3 mg Chl a/m3 = (Ca * v) (V * p) v = 10mL (acetone + SW) V= 1 mL (SW) p = 1.6 (path length)

3. Amount in 5 mL homogenate?
We have mg / m cm / m
106 cm3 / m cm3 = mL mL / m3
Now we have mg / 106 mL
Rearranging gives us mg / mL
Multiplying by 5 mL gives mg Chl a in the homogenate sample

4. We’re interested in the amount of Chl a in relation to the amount of zoox in the sample
Divide the mg Chl a just calculated by the zoox concentration determined from the hemocytometer
mg Chl a zoox