1. Molecular Cell Biology 5068
Bob Mercer, ;

2. Goals For MCB 5068
1. Obtain a solid foundation of knowledge in cell biology
2. Obtain a working knowledge of available techniques.
3. Be able to critically read and evaluate the scientific literature.
4. Be able to define and investigate a biological problem.

3. Molecular Cell Biology 5068
Lectures: Tuesday and Thursday, 8:30-10:00 AM, Cori Auditorium
Discussion Section: Wednesday, 3-4 PM
Papers assigned
One-page summary/critique expected

4. Molecular Cell Biology 5068
To Do:
Visit website:
Sign up for course.
Check out Self Assessment homework
Visit Discussion Sections: Read “Official” Instructions

5. Molecular Cell Biology 5068

6. Discussion Section Techniques
Protein purification using GST Fusion
CRISPER/Cas 9 gene editing
SDS-PAGE/Immunoblotting/Immunoprecipitation
Immunofluorescence
Sanger Sequencing
Real time PCR
Proliferation Assay/BrdU
In vivo intracellular pH determination/pHluorin
Size exclusion chromatography

7. TA’s: Kelsy Cotto Kelsy.cotto@wustl.edu Bisiayo Fashemi

8. Grading: 3 in class closed book exams accounting for 75% of grade
25% of final grade based on performance in Discussion Section
Last years final grades:
28 A
33 A-
30 B+
13 B
1 B-
1 C+