1. H-NS2 protein interacts with Lon and H-NS protein in vitro.
H-NS2 protein interacts with Lon and H-NS protein in vitro. (A and B) Analysis by SDS-PAGE and Coomassie blue staining of fractions from pulldown experiments after incubation of H-NS2 containing a His tag either at the C-terminal end (A) or N-terminal end (B) with a cellular extract from E. coli 042. Lanes 1 and 2 correspond to wash fractions with 50 mM imidazole. Lanes 3 to 5 correspond to elution fractions with 200 mM imidazole. Lane 6 corresponds to the elution fraction with 500 mM imidazole. Lane 7 corresponds to the elution fraction with 1 M imidazole. Bands indicated by black stars were identified by LC/MS-MS and correspond to Lon protein in panel A and H-NS protein in panel B. Experiments were repeated three times. The results of a representative experiment are shown.
A. Prieto et al. mSystems 2018; doi: /mSystems