1. Metabolism Lab Part 1: cellular respiration Aerobic respiration:
C6H12O6 + __  CO2 + H2O + Energy
Anaerobic respiration:
C6H12O6  nitrates/sulfates + CO2 + Energy
(e.g., “_____ gas”)
Fermentation
Yeast: C6H12O6  _______ + Energy
Animals under oxygen deficit:
C6H12O6  ______ acid + Energy
Lactic acid is broken down when __ is available
All 3 process use glycolysis, which is anaerobic
But products differ

2. Respirometer C6H12O6 + O2  CO2 + H2O + Energy Which gas(es) go in?
mealworm
Which gas(es)
come out?

3. Add soda lime to _______ CO2 Now gas movement is primarily inward
mealworm

4. Movement of meniscus reflects oxygen consumption
mealworm

5. Measuring oxygen consumption
1 ml
pipette
0.1 ml

6. Respiration procedures
Tare scale
Follow instructions on pgs 52-55
Pose hypotheses before conducting tests; pg 63

7. Ending measurement of one trail is the beginning
From pg 59:
Ending measurement of one trail is the beginning
measurement of the next trial
0.35
0.33
0.37
0.35

8. Expected values for calculated metabolic rate (G):
10.00 –>
If your calculated values are outside this range
you’ve probably miscalculated.
The point of questions is to compare your daily
(24 hr) caloric intake to that of a single pea or worm.

9. “warm blooded” versus “cold blooded”
Terms are misleading
Snakes are cold blooded, right?
What if the snake has been basking on a hot rock?
Two issues here:
Whether heat is generated from within the body
Whether blood temperature changes with outside
temperature

10. Better terms:
Endotherm: body heat generated ______
E.g., mammals: requires ~70% of food energy
Ectotherm: body heat absorbed from _______
E.g., reptiles: require 10x less food energy
Homeotherm: keep internal body temp. _______
E.g., mammals
Poikilotherm: internal temp. ______ with outside
E.g., most fish

11. Pound for pound, smaller animals need ____ energy
Elephant
0.1 kg
elephant tissue
Mouse
0.1 kg
mouse tissue

12. Some vocabulary terms for experimental science
Dependent variable: the variable that is expected to
change as other factors are adjusted.
Independent variable: variable(s) that is/are not
influenced by experimental treatments.
Experimental treatment: the factor whose
influence is being tested. Usually administered at a
range of levels.
Experimental control: an experimental setup in which
the treatment or condition is not applied. Used to
confirm that outside factors do not influence results
of the experiment.

13. Part 2: Photosynthesis
Light Energy + H2O + CO2  C6H12O6 + O2
Essentially the opposite of cellular respiration:
C6H12O6 + O2  CO2 + H2O + Energy
How might we experimentally increase photosynthesis?
Prepare test tubes as described on page 56