Presentation is loading. Please wait.

Presentation is loading. Please wait.

Adaptamers Thrombin-binding domain Streptavidin-binding domain

Published byKatariina Salonen Modified over 6 years ago

Similar presentations

Presentation on theme: "Adaptamers Thrombin-binding domain Streptavidin-binding domain"— Presentation transcript:

1 Adaptamers Thrombin-binding domain Streptavidin-binding domain
Linking region Thrombin Streptavidin

2 Why develop adaptamers?
To offer a new way to target substrates to cells. To create a tool for studying cell-cell interactions. To provide a foundation for engineered catalysts. happy colorful things.

3 Adaptamer testing Bead surface Adaptamer = A15 Control: no thrombin
streptavidin adaptamer Adaptamer = A15 thrombin anti-thrombin antibody Control: no thrombin Bead surface

4 Adaptamer quenching… + + Results: Displacement strand
change color of displacement strand, stop blinding pp (yellow) No displacement strand added Displacement strand added Background fluorescence +

Download ppt "Adaptamers Thrombin-binding domain Streptavidin-binding domain"

Similar presentations

Serological tests (Antigen antibody interactions)

Serological tests (Antigen antibody interactions)
(DNA-based) cell targeting. Overview Goals 3 designs Possible Implementation Future directions.

(DNA-based) cell targeting. Overview Goals 3 designs Possible Implementation Future directions.
Cell Surface Targeting 8/21/06. Adaptamers A20 A35 A50 Link-aptamer T35 S35 S50 T20 S20 T50 A15.

Cell Surface Targeting 8/21/06. Adaptamers A20 A35 A50 Link-aptamer T35 S35 S50 T20 S20 T50 A15.
Cell Separation Using Magnetic Beads

Cell Separation Using Magnetic Beads
Antigens & Antibodies: reactions, detection, and applications.

Antigens & Antibodies: reactions, detection, and applications.
ELISA Assay. What Is It? Enzyme immunoassay (EIA) is a test used to detect and quantify specific antigen-eliciting molecules involved in biological processes,

ELISA Assay. What Is It? Enzyme immunoassay (EIA) is a test used to detect and quantify specific antigen-eliciting molecules involved in biological processes,
SOLAR ENERGY CARS. SOLAR ENERGY CARS Our project targets to fight against pollution. The whole world does not pay attention to the planet. There is a.

SOLAR ENERGY CARS. SOLAR ENERGY CARS Our project targets to fight against pollution. The whole world does not pay attention to the planet. There is a.
The focus here is on best techniques for distinguishing strains of the same species.

The focus here is on best techniques for distinguishing strains of the same species.
E-beam Size-Dependent Self- Assembly Protein Array.

E-beam Size-Dependent Self- Assembly Protein Array.
Immunological Tests A harmful agent can be detected

Immunological Tests A harmful agent can be detected
Detection of proteins on slide preparations Histochemistry.

Detection of proteins on slide preparations Histochemistry.
200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 Adding SubtractingMultiplyingDividingMiscellaneous.

Adding SubtractingMultiplyingDividingMiscellaneous.
Cell Surface Targeting

Cell Surface Targeting
Biochemical instrumental analysis - 12 Dr. Maha Al-Sedik 2015 CLS 332.

Biochemical instrumental analysis - 12 Dr. Maha Al-Sedik 2015 CLS 332.
BioIntelligence Lab Reaction discovery enabled by DNA-templated synthesis and in vitro selection Matthew W. Kanan, Mary M. Rozenman, Kaori Sakurai, Thomas.

BioIntelligence Lab Reaction discovery enabled by DNA-templated synthesis and in vitro selection Matthew W. Kanan, Mary M. Rozenman, Kaori Sakurai, Thomas.
Cell Surface Targeting 7/31/06. Last Time Identified two potential problems with our procedure –Use human thrombin, not bovine! –Denature aptamers!

Cell Surface Targeting 7/31/06. Last Time Identified two potential problems with our procedure –Use human thrombin, not bovine! –Denature aptamers!
Cell Surface Targeting: The week of waiting 8/14/06.

Cell Surface Targeting: The week of waiting 8/14/06.
3:00. 2:59 2:58 2:57 2:56 2:55 2:54 2:53 2:52.

3:00. 2:59 2:58 2:57 2:56 2:55 2:54 2:53 2:52.
ELISA Enzyme-linked Immunosorbant Assay Detects the presence of minutes quantities of either an antibody or an antigen Important diagnostic tool in many.

ELISA Enzyme-linked Immunosorbant Assay Detects the presence of minutes quantities of either an antibody or an antigen Important diagnostic tool in many.
Cell Surface Targeting 8/7/06. Progress/agenda Streptavidin BioBricks –Sequencing for QuikChange-mutagenized single-chain dimer streptavidin showed 141bp.

Cell Surface Targeting 8/7/06. Progress/agenda Streptavidin BioBricks –Sequencing for QuikChange-mutagenized single-chain dimer streptavidin showed 141bp.

Similar presentations

Ads by Google