1. Supplemental data figure 1.
Assisting primer annealing by an anchor probe improves FLAG assay performance
Serial dilutions of target DNA were analyzed with the FLAG assay in presence (open diamonds) or absence (solid diamonds) of the anchor oligonucleotide. Each sample was tested in at least 6 replicates in 2 different runs. Mean threshold cycles (mean Ct) and standard deviations (SD) obtained are reported in the table. The equations shown refer to the correlation curves that interpolate the data points in presence or absence of the anchor oligonucleotide.