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Published byὍμηρος Μαλαξός Modified over 6 years ago
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Rotating Vessel Wall For the Adhesion of Cardiac Cells
Roli Kumar BME Oral Presentation#1 Advisor: Dr. Marc Lin
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Background Many surgical and radiational procedures have left areas of damaged tissue in the heart. Test new drugs study development and function of cardiac tissue
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Objectives 3-D cardiac tissue from a monolayer of cardiac cells
3-D cell cultures contain specifications similar to in vivo: cell to cell and cell to matrix interactions composition of interstitial fluid Three dimensional Cultures of Prostatic Cells
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Design Specifications of Rotating Vessel
End over end mixing of the cells Continuous rotation of the vessel Max of .2 dyne/cm2 of shear stress Minimal fluid turbulence Bubble-free aeration eliminate hydrodynamic forces Solid Body Rotation Suspending the cells in a liquid medium.
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Apparatus Set-Up Plastic Rod Rotating vessel Water Magnetic Stirrer
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Work Completed Literature search was completed: Found a metal ball
Cell disassociation Rotating-Wall Vessel for Prostate Cells Found a metal ball
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Current Work I am meeting with Dr. Bader who does Cardiology research to learn how to disassociate and cardiac cell growth. Putting the apparatus together
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Future Plan of Action Calculate the sheer stress applied on cells when rotated Literature search on a way to bubble-free aeration of cells while they are rotating
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