1. 1-What matooke delivered the NARITAs?
WP1 DISCUSSION POINTS
1-What matooke delivered the NARITAs?
Tetriploids: Enzirabahima/Entukura, Kabucuragye, Nakawere, Nfuuka
Tetraploids: 222K-1, 917K-2, 1438K-1, 1201K-1, 401k-1, & 660K-1
2-What matooke delivered the new 48 selected hybrids?
Tetraploids: 365K, 197K-2, 1411K, 401K-1, 199K, 660K, 1154K

2. Emerging issues Influence of male parents on hybrids quality
Tetraploids giving good products
(Which tetraploids/diploids result into beer type of bananas)
Note: New tetrapliods are coming in

3. Emerging issues cont’d
How about aneuploids being used as parents?
Exchange of improved diploids among project partners – NARO/IITA/India
Increasing diversity of banana germplasm
Frequency of good hybrids when different males are used!
Analyse the importance of maternal grand fathers in producing good quality hybrids
When to stop making crosses (after maximising variability: use CV%, SD … to use Musabase

4. 3- All hybrid bananas from IITA and NARO to be evaluated in the same field at PYT stage
Replicated-multilocation (Kawanda, IITa sendus & IITa Arusha) trials are preferred
Considerations
Resources
Number of replications
Number of plants per genotype
Number of genotypes to be tested

5. Emerging issues Before disposal of trials (EET materials):
Archive the leaf/DNA samples of the phenotyped material are collected…. to contribute to the training populations
Protocol for conserving leaf samples
Composing a team to archive information
When to apply what we have e.g. markers from Nyine’s PhD work

6. 4- Need to reduce breeding blocks considering enough seeds & enough hybrids available
Yes/No
Rationalise crosses made and select suitable parents
Evaluate new materials getting into the programme to guide selection of material to retain
Eliminate parents not giving good products but conserve their genes/in form of leaves
More strict criteria for selection of hybrids in EETs
Use Musabase to make important decisions

7. 5- Is there a need to use genetic relationships as part of the crossing schemes? Covered!
6- Should EMBRAPA and IEB/IITA use same SSR?
-Yes, use same marker system
-To link with WP3

8. 7-What can WP2-5 offer now to WP1
WP2- Disease results – to Musabase
Germplasm screening to be coordinated, with standardised list-Musabase
WP3- Molecular marker results-
Wp4- Results of the survey on consumer preferences to adequately prepare for the incoming hybrids
WP5-functioning-usable

9. Action points
Develop protocol for leaf collection & archiving Robooni/Allan/Dalphine
Introduction of historical crosses data into Musabase: NARO & IITA Data-Excel/Note books/ Allan/Trusha (WP5)
Increased participation of WP2, 3 & 4 in Musabase
Disease screening results
Genotypes of breeding populations
Outcomes on information of consumer preferences

10. Action points cont’d Interaction with NRCB-India
-Information exchange (improved diploids)
Robooni to link with Backiyaran/Uma
Generation of info on male parents influencing quality of hybrids produced
Determine appropriate replications/locations for PYTs to make more effcient programme Allan/Robooni
Harmonising SSR markers used by IEB /IITA and EMBRAPA. Currently there is discrepancy among markers used by IITA/ IEB and EMBRAPA. To be discussed WP3