1. Group Name
MRSA2
Group member name
Karin Sundberg
Marc Nielsen
Ana Freitas
Maria Nummela
Jie Xu

2. Isolates Sample name Subtype Notes ERR070040-P12 ST2371
From Patient 12
ERR P24
From Patient 24
ERR P22
From Patient 22
ERR HW
From Health Care Worker - misses the ermC plasmid
ERR not-outbreak
ST8
Not part of the outbreak

3. Result of species identification (KmerFinder)
Strain
Species acc. to Harris et al 2013
Predicted species
Closest matching strain
Template coverage
ERR P12
S. aureus HO
97.43
ERR P24
97.47
ERR P22
97.36
ERR HW
ERR not-outbreak
USA_300_FPR3757
96.22

4. Representative KmerFinder result

5. Result of Multilocus Sequence Typing (MLST)
Strain
ST acc. to Harris et al 2014
Predicted ST
ERR P12
ST2371􀀃
ST-2371
ERR P24
ERR P22
ERR HW
ERR not-outbreak
ST8􀀃
ST-8

6. Representative Multilocus Sequence Typing result

7. Result of antibiotics resistance analysis
Isolate
Cefoxitin/Methicillin (MecA)
Cipro-floxacin (chromo-somal mutation)
Erythro-mycin (Erm(A), Erm(B), Erm(C))
Fusidic acid (chromo-somal mutation)
Gentamicin (aac(6’)-aph(2''))
Mupirocin (chromo-somal mutation)
Rifampicin (chromo-somal mutation)
Tetra-cycline (tetC, tetM, tetR, tetS
Vancomycin (Chromo-somal mutations or the vanA or vanB gene cluster.)
Neomycin (aadD, aac(6')-aph(2''))
P12
RES
RES (norA)
RES (ermC)
Unknown
SUS
P24
P22
RES (ermc) HW
non-out-break
RES (ermA)
How is the agreement with the results presented in Harris et al 2013?
Problems with “Unknown”, can’t make conclusions on resistance caused by chromosomal mutations.

8. Result of plasmid analysis
Isolate
rep10
repUS12
rep20
rep5
rep7
ERR P12 2 27 13
ERR P24 31 72
ERR P22 10 25 65
ERR HW 40 52
ERR not-outbreak 3
For each strain write which node the replicon was found on. If not found, leave the cell empty.
Which plasmid is the ermC gene on? Rep10 (pDLK1-like plasmid)
The ermC gene is found on node 2 in P12, which also houses plasmid rep10. Similar for P22 (node 10).

9. Distance matrix made by NDtree and tree made with neighbour-joining

10. Distance matrix made by CSIPhylogeny and tree made with neighbour-joining