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Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes Irene Mondéjar, Ph.D., Luis A. Grullón, Ph.D., Francisco A. García-Vázquez, Ph.D., Raquel Romar, Ph.D., Pilar Coy, Ph.D. Fertility and Sterility Volume 97, Issue 2, Pages e3 (February 2012) DOI: /j.fertnstert Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 In vitro matured (SPZ−) and fertilized (SPZ+) (A, B, E, F) bovine and (I, J, M, N) porcine oocytes incubated with a primary antiplasminogen antibody and a secondary antibody conjugated to tetramethyl rhodamine isothiocyanate. Zona pelucida (ZP) was removed by pronase in some oocytes to better observe the antiplasminogen labeling at (C, D, G, H) oolemma and (K, L, O, P) ZP levels. Two pictures (transmitted and fluorescence light) are shown for each sample. Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 In vitro matured (SPZ−) and fertilized (SPZ+) (A-H) bovine and (I-P) porcine oocytes incubated with a primary anti-t-PA antibody, a secondary antibody anti-goat IgG produced in rabbit, and a tertiary antibody, anti-rabbit IgG produced in goat conjugated to tetramethyl rhodamine isothiocyanate. Zona pelucida (ZP) was removed by pronase in some oocytes to better observe the anti-t-PA labeling at oolemma and ZP levels. Two pictures (transmitted and fluorescence light) are shown for each sample. Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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Figure 3 In vitro matured (SPZ−) and fertilized (SPZ+) (A-H) bovine and (I-P) porcine oocytes incubated with a primary anti-u-PA antibody, a secondary antibody anti-goat IgG produced in rabbit, and a tertiary antibody, anti-rabbit IgG produced in goat conjugated to tetramethyl rhodamine isothiocyanate. Zona pelucida (ZP) was removed by pronase in some oocytes to better observe the anti-u-PA labeling at oolemma and ZP levels. Two pictures (transmitted and fluorescence light) are shown for each sample. Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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